摘要
目的 研究L 精氨酸和L NAME对体外培养的牛小梁细胞增殖和凋亡的影响。方法 在体外培养的牛小梁细胞中分别加入浓度为 1× 10 -7mol/L、1× 10 -6mol/L、1× 10 -5mol/L、1× 10 -4 mol/L、1× 10 -3 mol/L及 1× 10 -2 mol/L的L 精氨酸和左旋 -硝基精氨酸甲酯 (L NAME)与小梁细胞共同孵育 48h ,以不加药组为对照组 ,用化学比色法、末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法 (TUNEL)及四甲基偶氮唑盐 (MTT)法 ,检测小梁细胞在上述药物中增殖和凋亡状况。结果 ≥ 1× 10 -4 mol/L的L 精氨酸通过促进NO的产生导致小梁细胞凋亡并抑制细胞的增殖 ;而≥ 1× 10 -5mol/L的L NAME通过抑制NO的产生 ,促进小梁细胞的增殖。结论 高浓度的NO可抑制小梁细胞的增殖 ,诱导细胞凋亡。
ObjectiveTo study the proliferation and apoptosis of cultured bovine trabecular meshwork(TM)cells influenced by L-arginine and L-NAME .MethodsTM cells with L-arginine and L-NAME were incubated for 48 h.In control group,no medicine was added.In treatment groups,concentrations of L-arginine and L-NAME were 1×10 -7 mol/L,1×10 -6 mol/L,1×10 -5 mol/L,1×10 -4 mol/L,1×10 -3 mol/L,1×10 -2 mol/L,respectively.NO 2 -in supernate,the proliferation and apoptosis of TM cells were detected by Griess assay,terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) and MTT assay.Result≥1×10 -4mol/L of L-arginine could induce the apoptosis of TM cells and inhibit the proliferation of TM cells by promoting the synthesis of NO.However,≥1×10 -5mol/L of L-NAME could accelerate the proliferation of TM cells by inhibiting NO product.Conclusion Nitric oxide in high concentration can induce TM cell apoptosis and suppress cell proliferation.
出处
《眼科研究》
CAS
CSCD
北大核心
2002年第5期401-403,共3页
Chinese Ophthalmic Research
