摘要
目的探讨一氧化氮（NO）在继发性脊髓损伤中的作用。方法通过伤前30min大鼠蛛网膜下腔注射NO合成酶底物左旋精氨酸（L－Arg）及其抑制剂（L－NAME），采用激光多普勒血流仪观测NO对伤段脊髓血流（SCBF）的影响，并评估NO对继发性脊髓损伤的作用。结果L－Arg改善了伤段SCBF，但加重了脊髓损伤。适当剂量的L－NAME降低了早期的SCBF，却改善了神经功能。然而大剂量的L－NAME由于长时间的抑制了NO的产生，导致了脊髓长时间的缺血，不利于神经功能的恢复。结论NO的过度释放加重了继发性脊髓损伤，而过度抑制也同样不利于神经功能的恢复。
Objective To explore the effect of NO on secondary spinal cord injury.Method L－arginine{L－Arg} and N－nitric－L－arginine methylester{L－NAME}were infused into subarachnoid space of rats 30 min before spinal cord injury(SCI).Spinal cord blood flow(SCBF) at trauma sites was monitored.After SCI by a Laser－Doppler flowmetry and neurological function was evaluated.Result The result showed that L－arg increased SCBF at trauma sites,but exaggerated the SCI.The proper dose of L－NAME decreased early SCBF at trauma sites,but ameliorated neural function.Large dose of L－NAME restricted NO production for a long time and resulted in spinal cord ischemia,thus exacerbating SCI.Conclusion That over－production or over－restriction of NO worsen SCI.
出处
《中国组织工程研究》
CAS
CSCD
2001年第7期40-41,共2页
Chinese Journal of Tissue Engineering Research
关键词
一氧化氮
脊髓损伤
脊髓血流
nitric oxide
spinal cord injury
spinal cord blood flow
