摘要
目的 探讨大鼠脊髓损伤后两种结构型一氧化氮合酶（ cNOS） mRNA表达的变化。方法 以 Nystrom方法建立大鼠脊髓压迫伤模型，用逆转录聚合酶链反应（ RT－ PCR）法测定伤段脊髓组织神经型及诱导型 NOS（ nNOS， eNOS） mRNA的表达情况。结果 脊髓压迫伤后 nNOS mRNA及 eNOS mRNA表达均增强，伤后 6 h达到高峰， eNOS mRNA表达增强更明显。结论 脊髓损伤后结构型 NOS mRNA的表达主要在伤后早期增强，不同的结构型 NOS mRNA表达增强的程度不同。
Objective To study the changes of constructive nitric oxide synthase (cNOS) gene expression in injured spinal cord tissue. Method Establish a compression injury model of rat spinal cord and gene expression levels of neuronal NOS (nNOS) and endothelial NOS (eNOS) are measured by reverse transcription－ polymerase chain reaction (RT－ PCR).Result Expression of nNOS mRNA and eNOS mRNA were upregulated after injury,reaching the maximum 6 h after injury, while increase of the expression of eNOS mRNA was especially significant. Conclusion cNOS gene expression increased,especially in early stage, after spinal cord injury , and expression of eNOS mRNA is higher than that of nNOS mRNA.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
2001年第16期36-37,共2页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金资助课题( 39800166)
关键词
脊髓损伤
一氧化氮
一氧化氮合酶
基因表达
spinal cord injuries
nitric oxide
nitric oxide synthase
gene expression
