摘要
目的：观察微囊化异种坐骨神经组织细胞移植对脊髓损伤大鼠降钙素基因相关肽（CGRP）表达的影响.方法：取家兔预变性处理1周的坐骨神经制成组织细胞悬液,与1.5%海藻酸钠溶液混合并喷入20mmol/L氯化钡溶液中制成微囊化的组织细胞悬液.将其植入SD大鼠脊髓损伤处,通过免疫组织化学显色观察CGRP的表达情况.结果：SCI后各组大鼠右侧脊髓前角CGRP阳性细胞数减少,1周后均不同程度地逐渐恢复.SCI后1周,细胞组和微囊组间右侧脊髓前角CGRP阳性细胞数差异无统计学意义,但两者较损伤组差异有统计学意义;SCI后2、4、8周,与损伤组和细胞组比较,微囊组右侧脊髓前角CGRP阳性细胞数增多.结论：微囊化异种坐骨神经组织细胞移植促进了大鼠损伤脊髓CGRP的表达,有利于脊髓神经元的再生.
Objective： To observe the effects of xenotransplantation of the microencapsulated sciatic nerve tissue/cells on the expression of calcitonin gene-related peptide （CGRP） after spinal cord injury （SCI） in rats. Methods： Bilateral sciatic nerves of rabbits were preconditioned for 1 week were dissected under sterile conditions and made into nerve tissue/cells suspension which was mixed with 1.5% sodium alginate solution. The mixture was then extruded using a droplet generation device into 20 mmol/L barium chloride solution to form barium-alginate capsules. Microencapsulated nerve tissue/cells suspension was implanted into the injured sites of the spinal cord in rats. By immunohistochemstry, the expressions of CGRP were observed. Results ： The number of CGRP-positive cells in the right ventral horn of spinal cord of rats reduced remarkably in each group after SCI, and it gradually recovered in varying degrees 1 week later. The difference of the number of CGRP-positive cells between the microcapsule group and the cell group was not remarkable, but both of them existed in the distinction when compared with the injury group （P 〈0.05）. At 2, 4 weeks and 8 weeks after SCI, compared to the cell group and injury group, the number of CGRP-positive cells in the microcapsule group increased obviously. Conclusion： The xenotransplantation of the microencapsulated sciatic nerve tissue/cells promotes the expression of CGRP after SCI in rats, benefiting the regeneration of neuron.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2011年第2期156-159,175,共5页
Chinese Journal of Anatomy
基金
国家自然科学基金（30060034）
江西省教育厅科技基金（GJJl0350）
江西省卫生厅科技基金（20081098）
关键词
微囊
异种移植
降钙素基因相关肽
施万细胞
脊髓损伤
microcapsule
xenotransplantation
calcitonin gene-related peptide
Schwann cells
spinal cord injury
