摘要
通过Nystrom脊髓后路压迫模型（35．0g／10min）造成脊髓中度损伤，应用激光多普勒血流仪观测了大鼠伤前30min，蛛网膜下腔注射生理盐水及亚硝基左旋精氨酸甲酯（L－NAME）0．15mg、1．5mg三组动物伤后局部脊髓血流的动态变化，并评定了伤后4周神经功能恢复情况。结果发现，L－NAME0.15mg短时间内抑制了脊髓血流，而L－NAME1．5mg较长时间抑制了脊髓血流。4周后L－NAME0．15mg组脊髓功能优于盐水组，而L－NAME1．5mg组脊髓功能较盐水组差。结果提示，适量的L－NAME由于短时间限制了一氧化氮（NO）的释放，有利于神经功能恢复；大剂量的L－NAME由于持续抑制了NO释放而致脊髓损伤加重。
In this experiment, the spinal cord compression model reported by Nystrom was used to result in moderate spinal cord injury (35. 0g/10min) in rats. Saline L-NAME 0. 15mg or 1. 5 mg was infused into the spinal subrachnoid space in three groups of rats respectively at 30min before spinal cord injury. The change of spinal cord blood flow (SCBF) at injury sites was monitored by way of Laser-Doppler flowmetry for four hours, and the neurological function of injuried rats was evaluated four weeks after spinal cord injury. The result showed that the duration of decrease in SCBF of the group of 0. 15mg L-NAME was shorter than that of 1. 5mg L-NAME group. After 4 weeks, the neurological function of rats of .15mg L-NAME was better than that of rats of saline control and 1. 5mg L-NAME.But the neurological function of injected rats of 1. 5mg L-NAME was worse than that of saline injected rats. The experimental results indicated that moderate L-NAME could ameliorate. acute spinal cord injury, and a large dose of L-NAME could result in spinal cord long-time ischemia and worsen acute spinal cord injury.
出处
《中国脊柱脊髓杂志》
CAS
CSCD
1997年第3期119-122,共4页
Chinese Journal of Spine and Spinal Cord
关键词
脊髓损伤
脊髓血流
一氧化氮合成酶
L-NMAE
Spinal cord injury
Spinal cord blood flow
N-nitro-L-arginine methyl ester
Nitric oxide synthase
