摘要
目的 研究梁金菇多糖 (LJPS)对造血功能辐射损伤的治疗作用及机理。方法 用造血祖细胞克隆法 ,内源性脾结节形成法 ,骨髓有核细胞计数及脾脏指数等方法观察了LJPS对6 0 Coγ射线照射 5 5Gy小鼠造血功能的影响。并用免疫组化法检测辐射骨髓及脾脏细胞的bcl 2蛋白的表达。结果 LJPS辐射后第 7天骨髓CFU GM、CFU E、CFU F数量增加 ,辐射第 14天的骨髓有核细胞数、脾脏指数、CFU S明显增多 ,辐射第 3、7、14天骨髓、脾脏bcl 2蛋白表达上升。结论 LJPS对小鼠造血功能辐射损伤具有治疗作用 ,其机理与调节骨髓、脾脏bcl
Objective\ To study the therapeutic effects of Liang Jin mushroom polysaccharide(LJPS)on hematopoietic function and its mechanism in radiation\|injured mice. Methods\ Colony\|forming ability of hematopoitic progenitors,colony\|forming unit\|spleen(CFU\|S),number of nucleated cells in bone marrow and spleen index were used to investigate the effects of LJPS on hematopoietic function of mice irradiated with 5.5 Gy 60 Co γ\|rays and immunohistochemical technique was used to detect the bcl\|2 protein in murine bone marrow and spleenic mononuclear cells. Results\ LJPS could markedly enhance the prolifertion of hematopoietic progenitors and stromal cells in bone marrow of mice irradiated with 5.5 Gy 60 Co γ\|rays.On the 14th day after irradiation,compared with the negative control group,CFU\|S, spleen index,number of nucleated cells in bone marrow increased markedly in LJPS group,On days 3,7 and 14,bcl\|2 protein level elevated markedly in the LJPS group. Conclusion LJPS has therapeutic effects on irradiated hematopoietic system in mice,and its mechanism is associated with up\|regulation of bcl\|2 protein in BM and spleen.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2002年第4期291-293,共3页
Chinese Journal of Radiological Medicine and Protection
基金
甘肃省科委重点基金资助项目 (GS981 A43 14 9)
