摘要
EGCG是绿茶中提取分离出的生物活性物质 ,是一类具有开发前景的天然产物。将绿茶提取物或茶多酚先经乙酸乙酯、氯仿两种试剂多次富集和脱除咖啡因和色素 ,得儿茶素总含量不低于 95 %的茶多酚 ,再经Sephadex L H-2 0柱层析纯化 ,得 EGCG单体 ,用 ESI-MS和 HPLC等方法鉴定和验证了其化学结构 ,其纯度均大于 96.7% ,得率为 2 3 .86% ,回收率为 71.2 5 %。
EGCG is a biologic active substance extracted from green tea, and has large exploitation foreground of natural product. The high Purification catechins was enriched at least 5 times by A and B reagents to obtain and taken off caffeine and pigments, whose total content was determined to be above 95% by HPLC. The catechins complex obtained by the above methods was separated and purified by Sephadex LH-20 Column Chromatography to obtain EGCG monomer, whose Purification was over 96%,yield and recycling rate were 23.86% and 71.25% respectively. The molecular structure of EGCG was further confirmed by MS methods.
出处
《茶叶》
2002年第3期136-137,144,共3页
Journal of Tea
