摘要
目的检测上皮间质转化相关蛋白(N-cadherin,E-cadherin,vimentin,SMA,CK19)在胆道闭锁(biliary atresia,BA)患儿肝组织中的表达情况,初步探讨其在BA患儿肝脏上皮间质转化中的意义。方法采用免疫组化法对34例BA患儿(实验组)及13例胆总管囊肿患儿(对照组)的肝组织标本进行上皮间质转化相关蛋白检测,分析其表达差异及其与肝纤维化严重度的相关性。结果实验组与对照组相比,N-cadherin、E-cadherin、vimentin、SMA、CK19抗体不同表达等级的构成比存在统计学差异(P <0. 05)。结论上皮间质转化相关蛋白在BA患儿肝组织中的表达水平总体高于胆总管囊肿患儿,提示上皮间质转化相关蛋白可能参与BA患儿肝纤维化的发生与发展。
Objective To detect the expressions of N-cadherin E-cadherin vimentin SMA and CK19 inbiliary aressia and congenital cyst of common bile duct and discuss the relationship with in epithetialtomesenchymal transition. Methods The expression of N-cadherin,E-cadherin,vimentin,SMA and CK19 in biliaryatresia （n = 34） and common bile duct cyst （n = 13） were detected by immunohistochemistry SP method. Results The expression ratios of N-cadherin. E-cadherin,vimentin,SMA and CK19 were higher in liver of bileduct than those in common bile duct （P 〈 0. 05）. Conclusion The expressions of N-cadherin,vimentin,SMAand CK19 are closely correlated with epithelialtomesenchymal transition. And EMT may be one of importantcauses of hepatic cirrhosis.
作者
吴艳
杨文萍
张守华
徐红艳
曾松涛
黄慧
熊枫
曾华
Wu Yan;Yang Wenping;Zhang Shouhua;Xu Hongyan;Zeng Songtao;Huang Hui;Xiong Feng;Zeng Hua(Department of Pathology,Jiangxi Children＇s Hospital,Nanchang 330006,China;Department ofGeneral Surgery,Jiangxi Children＇s Hospital,Nanchang 330006,China)
出处
《临床小儿外科杂志》
CAS
2018年第11期826-829,共4页
Journal of Clinical Pediatric Surgery
基金
江西省卫生计划与生育委员会科技计划(编号:20155586)
国家自然科学基金项目(编号:81460118)
关键词
胆道闭锁
肝硬化
免疫组织化学
研究
Biliary Atresia
Liver Cirrhosis
Immunohistochemistry
Research
