摘要
目的探讨胆道闭锁患儿肝组织中MMP-1、MMP-2、TIMP-1的表达及与胆道闭锁预后的关系。方法收集19例胆道闭锁患儿的临床资料和肝脏组织标本,依据Ohkuma’s分级标准将肝组织纤维化程度分为Ⅰ～Ⅳ级。采用免疫组化方法检测肝脏组织MMP-1、MMP-2、TIMP-1的表达,通过图像分析技术进行定量分析。结果根据临床疗效将患儿分为预后良好组(7例)和预后不良组(12例)。预后良好组与预后不良组接受手术时的平均日龄分别为58.57±9.32d、122.25±54.67d,两者比较,差异有显著统计学意义(P<0.01)。预后良好组肝纤维化程度较预后不良组轻(P<0.05)。预后不良组MMP-2、TIMP-1的表达明显高于预后良好组(P<0.01),而MMP-1在两组中的表达差异无统计学意义(P>0.05)。结论胆道闭锁肝组织MMP-2、TIMP-1的表达与病人预后有关,其高表达预示患儿肝脏纤维化进展快,预后较差。
Objective The expressions of MMP-1,MMP-2,TIMP-1 were observed in different prognostic groups after Kasai procedure . Methods The clinical data and liver specimens of nineteen biliary atresia （BA） were collected. Hepatic fibrosis was classified into Ⅰ to Ⅳ grades according to Ohkuroa＇s standard. The immunohistochemichal method was used to detect the expressions of MMP-1,MMP-2,TIMP-1.The image analysis technology was used to quantitatively analyze the protein expression. Results BA children were divided into favorable prognosis group （FPG） and unfavorable prognosis group （UFPG）, according to their outcome after Kasai procedure. There were seven and twelve patients in FPG and UFPG, respectively .The average day-age at operation were 58.57 ± 9.32; 122.25 ± 54.67 in FPG and UFPG, respectively. There was obvious difference in age between two groups （P 〈 0.01）. The hepatic fibrosis in UFPG was more serious than that in FPG （P 〈 0.05）. The expressions of MMP-2,TIMP-1 were markedly higher in UFPG than in FPG （P 〈 0.01）, there was no difference in MMP-1 expression between two groups （P 〉 0.05）. Conclusions MMP-2 and TIMP-1 play an important role in the procession of liver damage and fibrosis in BA, their overexpression is related to rapid hepatic fibrosis and poor outcome of BA.
出处
《临床小儿外科杂志》
CAS
2007年第1期5-8,共4页
Journal of Clinical Pediatric Surgery
