摘要
目的提高海洋小单孢菌FIM02523发酵液中的生物活性成分rakicidin B含量。方法采用单因素实验、正交设计实验等方法优化发酵培养基组成比例及培养条件。结果确定了最优rakicidin B发酵培养基:可溶性淀粉4.0%,蔗糖1.0%,大豆蛋白胨1.0%,酵母粉2.0%,甘氨酸0.1%,NaCl 0.5%,MgSO4 0.05%,CaCO3 0.5%;最优摇瓶发酵条件:发酵周期为120h,接种量5.0%,500mL摇瓶装量100mL,摇床转速220r/min,发酵温度30℃,培养基初始pH值为7.5。结论优化后发酵工艺rakicidin B的发酵效价较初始工艺提高了约7.3倍。
Objective To enhance the productivity of rakicidin B by marine Micromonospora sp. FIM02523. Methods Single-factor experiment and Orthogonal experimental design were applied to optimize the fermentation medium and fermentation conditions. Results Components of the optimized medium were soluble starch 4.0%, sucrose 1.0%, soybean peptone 1.0%, yeast powder 2.0%, glycine 0.1%, NaCl 0.5%, MgSO4 0.05% and CaCO3 0.5%; The optimum fermentation conditions were ascertained, fermentation time 120 hours, inoculation 5%, 80 mL medium in 500 mL flask, rotation speed 220 r/min, culture temperature 30 ℃, pH 7.5 before sterilization. The fermentation titer of rakicidin B could reato approximately 7.3 times higher than that of the original.
作者
周剑
林如
欧阳浩亮
连云阳
林风
江红
Zhou Jian;Lin Ru;Ou-yang Hao-liang;Lian Yun-yang;Lin Feng;Jiang Hong(Fujian Key Laboratory of Screening for Novel Microbial Products,Fujian Institute of Microbiology,Fuzhou 35000)
出处
《中国抗生素杂志》
CAS
CSCD
2018年第11期1397-1402,共6页
Chinese Journal of Antibiotics
基金
福建省公益类科研院所省重点项目(No.2017R1009-14)
国家新药重大专项(No.2017ZX09101003-007-007和No.2017ZX09201001-002-005)
关键词
发酵
培养基优化
正交设计
Rakicidin
B
Fermentation
Medium optimization
Orthogonal experimental design
Rakicidin B
