摘要
为研究原发性肝细胞性肝癌中nm23-H1和PTEN的表达状况，并分析两者的临床意义，本研究选取顺德第一人民医院2005年1月至2008年12月原发性肝细胞性肝癌手术患者蜡块标本68例，按Edmondson标准进行组织学分级，68例原发性肝细胞性肝癌组织为对照组，5例正常肝组织作为参照组。通过采取免疫组化S-P实验法测定两组标本nm23-H1、PTEN的表达。结果显示，nm23-H1蛋白在HCC、正常肝组织中的阳性表达率分别是30．88％（21168）、86．67％（13／15），nm23-H1在HCC中的表达低于正常肝组织（χ^2=15．813，p〈0．05）。同时，根据实验分析可见，PTEN在正常肝组织、肝癌组织中的阳性率分别为93．33％（14/15）、60．29％（41／68），且PTEN在HCC中的表达低于正常肝组织（χ^2=6．001，p〈0．05），两者均与肿瘤的分化程度和转移相关。本研究表明PTEN、nm23-H1与HCC侵袭性强弱和是否发生转移有一定的相关性。
In order to study the expression ofnm23-H1 and PTEN in primary hepatocellular carcinoma, and to analyze the clinical significance of the two, this study selected 68 patients with primary hepatocellular carcinoma from January 2005 to December 2008 who were enrolled in the Shunde First People＇s Hospital. The specimens were classified according to Edmondson criteria. 68 cases of primary hepatocellular carcinoma were selected as control group and 5 cases of normal liver tissue as reference group. The expression ofnm23-H1 and PTEN in the two groups was determined by immunohistochemical S-P assay. The results showed that the positive expression rates ofnm23-H1 protein in HCC and normal liver tissues were 30.88% （21/68） and 86.67% （13/15） respectively. The expression of nm23-H1 in HCC was lower than that in normal liver tissues （χ^2= 15.813, p〈0.05）. Meanwhile, according to experimental analysis, the positive rates of PTEN in normal liver tissues and liver cancer tissues were 93.33% （14/15） and 60.29% （41/68）, respectively. The expression of PTEN in HCC was lower than that of normal liver tissue （χ^2=6.001, p〈0.05）, both of which were related to tumor differentiation and metastasis. The result showed that PTEN, nm23-H1 and HCC had a certain correlation with the strength of invasive and metastasis.
作者
王晓蔚
康凯夫
张鑫
Wang Xiaowei;Kang Kaifu;Zhang Xin(Yuncheng Vocational Nursing College,Yuncheng,044000;Shunde Hospital of Southern Medical University,Foshan,528300)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2018年第11期4949-4954,共6页
Genomics and Applied Biology
基金
广东省社会发展领域科技计划项目(No.200883113)资助
