摘要
目的了解2017年陕西省韩城市利什曼病流行现状,为制定疫情监测方案及防治措施提供依据。方法通过中国疾病预防控制中心疾病监测信息报告管理系统,收集2017年韩城市利什曼病疫情信息和调查报告,采集患者静脉血。选择韩城市2012年以来有病例报告的桑树坪(王峰)镇程家洞村、杨湾村和水草塔村,西庄(盘龙)镇曹家山村,昝村镇东贾村,龙门镇西塬村等6个村为调查点,其中程家洞村、杨湾村、水草塔村、曹家山村为丘陵区生境,西塬村和东贾村为黄土台原地区生境。在调查点内以人工小时捕捉法从6月中旬至9月下旬连续监测白蛉密度。以既往病例家庭为线索,随机采集人群、家犬EDTA-K2抗凝血及普通血样各3 ml,采集疑似病犬的骨髓,血清学抗体阳性犬捕杀后采集骨髓拭子。人群血清采用rK39免疫层析试纸条检测利什曼原虫抗体;犬血清以ELSIA试剂盒检测利什曼原虫抗体,血清学抗体阳性犬利用rK39免疫层析试纸条进行复检。家犬骨髓进行病原学检测。提取患者和血清学抗体阳性犬骨髓和阳性白蛉组织DNA, PCR检测利什曼原虫动基体靶基因,分别扩增内转录间隔区1 (ITS-1)、小亚单位核糖体核糖核酸(SSU rRNA)、 DNA重复序列并测序,进行聚类分析,鉴定虫种。结果韩城市2017年共报告10例利什曼病,其中8例为外来务工人员, 2例为本地儿童。人群利什曼病血清抗体阳性率为3.1%(7/224),无症状人群PCR未检出阳性。犬利什曼原虫血清抗体阳性率为34.3%(115/335),丘陵区犬血清抗体阳性率51.5%(105/204)高于黄土台原地区的7.6%(10/131)(χ~2=68.000, P <0.05);媒介监测, 6-9月白蛉平均密度为16.7只/(人工·h),最高密度为122只/(人工·h),丘陵区平均密度23.6只/(人工·h),高于黄土台原地区的0.3只/(人工·h)(t=2.19, P <0.05)。共捕获白蛉336只,其中雌蛉168只,雄蛉168只。鉴定白蛉228只,其中中华白蛉占29.4%(67/228)。P
Objective To understand the current endemic status of visceral leishmaniasis in Hancheng City and provide basis for the establishment of endemic surveillance and control measures.Methods The endemic information and survey reports of visceral leishmaniasis in Hancheng City in 2017 were collected from the Disease Surveillance Reporting System of the Chinese Center for Disease Control and Prevention.Blood was collected from the patients.Six villages that have reported cases since 2012 were selected as the survey spots, including the Chengjiadong Village, Yangwan Village, and Shuicaota Village in Sangshuping Town, Caojiashan Village in Xizhuang Town, Dongjia Village in Zancun Town, and Xiyuan Village in Longmen Town.The former four villages located in the hilly regions while the latter two in the Loess plateau area.Based on the family information on disease incidence, anticoagulation and normal blood samples（3 ml each） were collected randomly from residents and domestic dogs.Bone marrow was collected from suspected dogs.Dogs with positive serological results were sacrificed to collect bone marrow swab.The sandfly density was monitored manually from middle June to late September.The rK39 immunochromatographic test strip and ELISA kit were used to detect the antibody for Leishmania in human serum and dog serum, respectively.The positive samples were further confirmed by rK39 immunochromatographic test strip.The dog bone marrow underwent pathological testing.DNA was extracted from blood, bone marrow and sandfly specimens.PCR was performed for kinetoplast target gene of Leishmania spp.The positive samples were further used for amplification of internal transcribed spacer 1（ITS-1） sequence, small subunit ribosomal RNA（SSU rRNA） and DNA repeats, and cluster analysis was made to identify the species.Result A total of 10 cases of visceral leishmaniasis in Hancheng City were reported in 2017, of whom 8 were workers from other cities and 2 were indigenous kids.The positive rate of visceral leishmaniasis among resid
作者
王凤萍
张铮
吕文
刘东立
王天海
王益群
王安礼
李胜振
石磊
马琳
曹磊
吴峰
WANG Feng-ping;ZHANG Zheng;LV Wen;LIU Dong-li;WANG Tian-hai;WANG Yi-qun;WANG An-Ji;LI Sheng-zhen;SHI Lei;MA Lin;CAO Lei;WU Feng(Shaanxi Provincial Center for Disease Control and Prevention,Xi＇an 710054,China;Hancheng City Center for for Disease Control and Prevention,Hancheng 715400,China;Wangfeng Town Hospital of Hancheng City,Hancheng 715400,China)
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2018年第5期483-488,共6页
Chinese Journal of Parasitology and Parasitic Diseases
基金
陕西省科技资源共享平台项目-公共卫生检测监测服务平台(No.2016FWPT-12)~~
关键词
利什曼病
流行病学调查
婴儿利什曼原虫
陕西
Visceral leishmaniasis
Epidemiologic investigation
Leishmania infantum
Shaanxi
