摘要
目的了解甘肃省迭部县犬利什曼原虫虫种类型和种系发育关系,为探索犬源型内脏利什曼病防控新方法提供依据。方法提取8份甘肃省迭部县洛大行政村无症状感染利什曼原虫犬血液样本DNA,采用PCR法扩增分离核糖体内转录间隔区1(ITS-1)基因片段,对扩增的目的片段进行基因测序;采用MEGA 7.0软件进行多序列比对,并经邻接法构建系统发育树,分析甘肃省迭部县犬利什曼原虫种系发育关系。结果8份无症状感染利什曼原虫犬血液样本均扩增出约320 bp的片段,与目标序列ITS-1大小一致。ITS-1序列比对显示,8份样本与婴儿利什曼原虫MG969403、MN648755虫株序列同源性为99.1%~100.0%;系统发育树显示,8份样本均与婴儿利什曼原虫聚为一支。结论甘肃省迭部县8份无症状感染利什曼原虫犬血液样本的原虫种型均为婴儿利什曼原虫。
Objective To investigate the species types and phylogenetic relationship of canine Leishmania in Diebu County,Gansu Province,so as to provide a basis for exploring new methods of prevention and control of canine visceral leishmaniasis.Methods DNA was extracted from blood samples of eight asymptomatic Leishmania-infected dogs in Luoda administrative village in Diebu County,Gansu Province.Ribosomal internal transcribed spacer 1(ITS-1)gene fragments were amplified and isolated by PCR,and then the amplified target fragments were sequenced.The MEGA 7.0 software was used for multiple sequence alignment,and a phylogenetic tree was constructed by neighbor-joining method to analyze the phylogenetic relationship of canine Leishmania in Diebu County,Gansu Province.Results Fragments of about 320 bp corresponding in size to the target sequence ITS-1 were isolated from all of the eight asymptomatic Leishmania-infected dogs blood samples.ITS-1 sequence alignment showed that the sequence homology between 8 samples and Leishmania infantum MG969403,MN648755 strains was 99.1%-100.0%;phylogenetic tree showed that all 8 samples were clustered into one branch with Leishmania infantum.Conclusion Leishmania infantum is identified from all of the eight asymptomatic Leishmania-infected dogs blood samples in Diebu County,Gansu Province.
作者
余大为
李凡
冯宇
杨成明
杨俊克
尕让东主
张永福
王成
Yu Dawei;Li Fan;Feng Yu;Yang Chengming;Yang Junke;Garangdongzhu;Zhang Yongfu;Wang Cheng(Department for Parasitic Disease Prevention and Control,Gansu Provincial Center for Disease Control and Prevention,Lanzhou 730020,China;Diebu Center for Disease Control and Prevention,Diebu 747400,China)
出处
《中华地方病学杂志》
CAS
北大核心
2022年第5期351-355,共5页
Chinese Journal of Endemiology
基金
国家自然科学基金(81703171)。
