摘要
目的了解新疆喀什地区人群利什曼原虫感染现状,为制定防治规划提供依据,并比较PCR和ELISA检测利什曼原虫无症状感染的效能。方法选取2009年9月新疆喀什地区黑热病流行严重的1县3乡4个调查点,采集黑热病患者及其家属和邻居的血液样品(每份血样分抗凝血和非抗凝血),采用ELISA检测血清中利什曼原虫特异性抗体;以RV1、RV2和KI3A、KI3B为引物,用PCR检测血样中利什曼原虫特异DNA片段。结果 PCR检测利什曼原虫特异DNA片段的阳性率为82.67%(62/75),ELISA检测利什曼原虫特异性抗体的阳性率为69.33%(52/75)。结论现阶段新疆喀什地区人群利什曼原虫感染率仍然颇高,尤其表现在与患者密切接触的患者家属和邻居;PCR是检测无症状利什曼原虫感染较敏感的方法 。
Objective To ascertain the current status of Leishmania donovani infection in the City of Kashi,Xinjiang,and provide a basis for practical policy-making.An additional aim was to evaluate the potentia1 for PCR and ELISA to detect L.donovani infection in humans.Methods Blood samples were collected in September 2009 from one county and three townships in the City of Kashi,Xinjiang.Each blood sample was divided into anticoagulant components and coagulant components.Antibodies against Leishmania in the samples were detected using ELISA,and the Leishmania-specific DNA in venous blood samples was detected using a PCR assay with two pairs of primers,RVI-RV2 and K13A-K13B.Results PCR positively detected Leishmania-specific DNA in venous blood samples at a rate of 82.67% while ELISA detected antibodies against Leishmania at a rate of 69.33%.Conclusion The prevalence of leishmaniasis in humans was rather high in the City of Kashi,Xinjiang Province.It was especially prevalent in people who were in close contact with infected individuals.A PCR assay proved to be a relatively sensitive method of detecting asymptomatic leishmaniasis infections in humans.
出处
《中国病原生物学杂志》
CSCD
2010年第11期844-847,共4页
Journal of Pathogen Biology
基金
科技部重大科技专项基金(No.2008IPB202)
中国医学科学院病原生物所资助项目
关键词
新疆
黑热病
感染率
ELISA
PCR
Xinjiang
kala-azar（visceral leishmaniasis）
infection
ELISA
PCR
