摘要
目的了解四川省的蚊、蠓以及相关虫媒病毒种类及分布。方法在自然界使用灯诱法采集吸血昆虫标本，形态学分类后分装，液氮保存备用。蚊虫、蠓虫研磨上清液接种BHK-21细胞和C6/36细胞进行病毒分离和病毒基因检测。使用BioEdit 7.0.5.3、MEGA 6.0等软件对病毒序列进行分子生物学特征分析。结果2016－2017年在四川省东南部3个县的7个自然村共采集到3属4种蚊虫17 019只，蠓虫12 700只。三带喙库蚊占蚊虫采集总数的79.4%（13 519/17 019），其次为骚扰阿蚊（11.1%，1 897/17 019），致倦库蚊占采集蚊虫标本总数的5.5%（930/17 019）；中华按蚊最少（4.0%，673/17 019）。蚊虫标本接种组织细胞后，在合江县采集的三带喙库蚊标本获得3株可以稳定传代的病毒分离株，经分子生物学实验鉴定为基因Ⅰ型流行性乙型脑炎（乙脑）病毒，在合江县采集的7批蚊虫中检测到乙脑病毒基因阳性。蠓虫标本接种细胞后未获得病毒分离物，在合江县采集的4批蠓虫标本经分子生物学检测，显示阿卡斑病毒基因阳性。结论三带喙库蚊是四川省东南部3县的优势蚊种，四川省东南部存在经蚊虫传播的乙脑病毒及以蠓虫为媒介的阿卡斑病毒。
Objective To investigate the distribution patterns of mosquitoes, midges and related arboviruses in Sichuan province.Methods Blood-sucking insects were collected from houses and pens, using the ultraviolet lights. Mosquito samples were classified according to morphologic characteristics and then stored at liquid nitrogen. All samples were incubated with BHK-21 and C6/36 cells for virus isolation and then detected for their viral genes. Sequences of the virus were identified and analyzed by molecular biological software, such as BioEdit 7.0.5.3, MEGA 6.0.Results In total, 17 019 mosquitoes from 3 genera and 4 species and 12 700 midges were collected from the southeast regions of Sichuan province in 2016 and 2017. Among them, 79.4% （13 519/17 019） belonged to Culex tritaeniorhynchus with 11.1% （1 897/17 019） as Armigeres subalbatus, 5.5% （930/17 019） were Anopheles sinensis and 4.0% （673/17 019） were Anopheles sinensis 3 virus strains that isolated from Culex tritaeniorhynchus were identified as typeⅠ Japanese encephalitis virus. Seven pools of mosquitoes isolated from Hejiang county were identified Japanese encephalitis virus gene positive through PCR amplification. With 4 pool midges were detected positive for Akabane virus through PCR gene amplification while midges samples didn’t have virus isolates.Conclusions Culex tritaeniorhynchus appeared the predominant species in the southeast regions of Sichuan. Japanese encephalitis virus transmitted by mosquitoes and Akabane virus by midges were prevalent in southeast Sichuan province.
作者
宋颂
付士红
周兴余
张佳珂
李伟
刘丽珺
李劲松
汪健
林于
李晓龙
何英
雷雯雯
王环宇
王斌
鲁晓睛
梁国栋
Song Song;Fu Shihong;Zhou Xingyu;Zhang Jiake;Li Wei;Liu Lijun;Li Jinsong;Wang Jian;Lin Yu;Li Xiaolong;He Ying;Lei Wenwen;Wang Huanyu;Wang Bin;Lu Xiaoqing;Liang Guodong(Qingdao University,Qingdao 266071,China;State Key Laboratory of Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Sichuan Province Center for Disease Control and Prevention,Chengdu 610041,Chin;Luzhou Municipal Center for Disease Control and Prevention,Luzhou 646000,China;Hejiang County Center for Disease Control and Prevention,Hejiang 646299,Chin;Nanxi District Center for Disease Control and Prevention,Yibin 644199,China)
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2018年第10期1381-1386,共6页
Chinese Journal of Epidemiology
基金
国家重点研发计划生物安全关键技术研发重点专项（2016YFCl201904）
国家自然科学基金（81290342）
关键词
流行性乙型脑炎病毒
阿卡斑病毒
蚊
蠓
Japanese encephalitis virus
Akabane virus
Mosquito
Midge
