摘要
用拉曼光谱原位分析姜黄橙黄色及黄色薄壁细胞中的物质。德宏、广西及西双版纳姜黄三种样品橙黄色细胞的拉曼光谱非常相似,较强峰出现在1 632/1 633/1 637、1 599/1 601/1 605、1 184/1 186/1 190 cm-1,中等强度的峰出现在1 529/1 528/1 534、1 425/1 426/1 430、1 306/1 308/1 311、1 235/1 235/1 239、1 167/1 168/1 173、1 122/1 125/1 130、967/969/975 cm-1。三种姜黄薄壁细胞中出现的强峰、次强峰位置、峰型都一致,说明三种姜黄橙色薄壁细胞中物质的主要成分相同。与姜黄素(curcumin)拉曼光谱的主要的19条谱线比较,在姜黄橙色细胞拉曼谱的17条谱峰中有16条与之对应。而黄色薄壁细胞中大部分谱线与支链淀粉的谱峰有较好的对应关系。用密度泛函理论计算了姜黄素的拉曼光谱,并对谱线进行了初步的归属。
In order to identify the ingredients in parenchymatous cells at room temperature,and avoid sample pretreatment and extractions which can be labor intensive,A method was presented that a novel and original approach to analyze in situ the main components of the parenchymatous cells in Curcuma longa L by means of Raman spectroscopy. Fresh samples were prepared by free-hand section. Under the DXR laser confocal micro Raman spectrometer,the parenchymatous cells can be seen with objective lens of 10. Dehong,guangxi,xisuabannan of Curcuma longa L,the three sample have similar Raman spectrum,high intensity bands are present at 1 632/1 633/1 637、1 599/1 601/1 605、1 184/1 186/1 190 cm^-1,middle intensity bands are present at 1 529/1 528/1 534、1 425/1 426/1 430、1 306/1 308/1 311、1 235/1 235/1 239、1 167/1 168/1 173、1 122/1 125/1 130、967/969/975. It means that the three samples have the same principal component. It has been found that the Raman spectrum of three sample correlate very well with curcumin Raman spectrum. There are 19 main spectroscopic bands in the Raman spectrum of curcumin. For the 17 presented spectroscopic bands of Curcuma longa L orange parenchymatous cells,there are 16 bands which correlate very well with curcumin. For Curcuma longa L yellow parenchymatous cells most bands correlate very well with amylopectin. The Raman spectroscopy of curcumin was calculated by density functional theory（DFT）. The Raman spectroscopic bands were given assignment with the help of calculation.
作者
司民真
李伦
张川云
张德清
SI Minzhen;LI Lun;ZHANG Chuanyun;ZHANG Deqing(Chuxiong Normal University a.Key Laboratory of Molecular Spectroscopy,Colleges and Universities in Yunnan Provinc;b.Application Institute of Spectroscopy Technology,Chuxiong Normal University,Chuxiong 675000,Yunnan,China)
出处
《激光生物学报》
CAS
2018年第4期332-337,共6页
Acta Laser Biology Sinica
基金
国家自然科学基金(11364001)
关键词
姜黄薄壁细胞
姜黄素
拉曼光谱
密度泛函理论
parenchymatous cells of Curcuma longa L
curcumin
Raman spectrum
density functional theory
