摘要
The chemical constituents of Cortex Moutan charcoal were studied in depth, and their coagulation activity was screened. The chemical constituents were isolated by polyamide, silica gel and Sephadex G-Sepharose chromatography purification, and their structures were identified by NMR spectroscopy and other analyses. A total of 10 compounds were obtained and identified as follows： 3-（2-furan）-l-（2-hydroxy-4-methoxy-phenyl）-2-propylene ketone （1）, 2-（2,5-hydroxy-4-methyl phenyl） propionate ethyl ester （2）, methyl tetradec-5-enoate （3）, 1,4-diethylcyclohexane （4）, catechol （5）, methyl-4-hydroxybenzoate （6）, 3-hydroxy- 2-methyl-4-pyrone （7）, 3,8-dihydroxy-2-metyl-chromone （8）, 3β-hydroxy-olean-12-en （9）, 1-monolinolein （10）. Among them, compounds 1 and 2 were new natural products, and 3-10 were isolated from the Cortex Moutan charcoal for the first time. The potential coagulation activities of compounds were evaluated, and compounds 2, 9 and 10 had strong hemostatic effects. While compounds 1 and 8 could significantly activate blood circulation.
对中药牡丹皮炭的化学成分进行深入研究,并对其凝血活性进行筛选。通过聚酰胺、硅胶、葡聚糖凝胶等柱色谱方法进行分离纯化,利用核磁共振波谱等方法鉴定结构。共分离鉴定了10个化合物,分别为3-(2-呋喃)-1-(2-羟基-4-甲氧基苯基)-2-丙烯酮(1)、2-(2,5-羟基-4-甲基苯基)丙酸乙酯(2)、5-十四烯酸甲酯(3)、1,4-二乙基-环己烷(4)、儿茶酚(5)、对羟基苯甲酸甲酯(6)、3-羟基-2-甲基-4-吡喃酮(7)、3,8-二羟基-2-甲基色酮(8)、3β-羟基-齐墩果烷-12-烯(9)、1-亚油酸单甘油酯(10)。其中1, 2为新天然产物, 3–10为首次从牡丹皮炭中分离得到。在体外评估化合物潜在的凝血活性,化合物2, 9和10具有强烈的止血作用,而化合物1和8可以活化血液循环。
基金
National Natural Science Foundation of China(Grant No.81473352)
Guangdong Province Innovation Training Project(Grant No.201610573040)
Guangzhou Science and Technology Bureau Foundation(Grant No.201707010170)
