摘要
目的观察人参皂苷Rh_2(G-Rh_2)对人胃癌SGC7901/ADR耐药细胞增殖、细胞周期及化疗敏感性的影响。方法 MTT法检测G-Rh_2与阿霉素(ADR)联用对SGC7901/ADR细胞增殖的影响,并计算逆转倍数(RF);流式细胞术检测G-Rh_2对SGC7901/ADR细胞周期的影响;蛋白印迹法检测G-Rh_2对SGC7901/ADR细胞P-糖蛋白(P-gp)、Bcl-2蛋白表达水平的影响。结果与ADR单药处理后细胞的半数抑制浓度(IC50)值(54.52μmol/L)比较,G-Rh_2与ADR联用时SGC7901/ADR细胞IC50值(30.14μmol/L)明显下降,RF为1.81;G-Rh_2与ADR联用能够将SGC7901/ADR细胞周期阻滞在G2/M期,与ADR单药处理比较,联用组细胞P-gp、Bcl-2的蛋白表达水平显著降低(P<0.05)。结论 G-Rh_2联合ADR能够提高SGC7901/ADR细胞的化疗敏感性,可能通过阻滞细胞周期、增加细胞凋亡进而抑制细胞增殖。
Objective To observe the effect of ginsenoside Rh2（G-Rh2） on the proliferation, cell cycle and chemotherapy sensitivity of human gastric cancer cell line SGC7901/ADR. Methods MTT assay was used to detected the effects of G-Rh2 and adriamycin（ADR） on the proliferation of drug-resistant SGC7901/ADR cells and to calculate the reversal fold（RF）. Flow cytometry was selected to detect The effects of G-Rh2 on cell cycle. the effects of G-Rh2 on the expression of P-gp and Bcl-2 proteins in drug-resistant SGC7901/ADR cells was detected by Western blotting. Results Compared with the IC50 value（54.52 μmol/L） after the ADR single drug treatment, the IC50 value（30.14 μmol/L） of the cells treated with G-Rh2 and ADR decreased significantly, and RF was 1.81. G-Rh2 combined with ADR arrested the cell cycle in G2/M phase and significantly decreased the protein expression of P-gp and Bcl-2（P〈0.05）. Conclusion G-Rh2 combined with ADR could increase the chemosensitivity of SGC7901/ADR cells, which may inhibit the proliferation of gastric cancer cells by blocking cell cycle and increasing apoptosis.
作者
胡双双
闫克敏
王娇娇
郭萌
帖君
聂勇战
肖海娟
HU Shuang-shuang;YAN Ke-min;WANG Jiao-jiao;GUO Meng;Tie Jun;NIE Yong-zhan;XIAO Hai-juan(Shaanxi University of Chinese Medicine,Xianyang 712046,China;Xijing Hospital of Digestive Disease,The Fourth Military Medical University,Xi＇an 710032,China;Department of Oncology,Hospital Affiiated to Shaanxi University of Chinese Medcine,Xianyang 712000,China)
出处
《中草药》
CAS
CSCD
北大核心
2018年第17期4113-4117,共5页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金重点资助项目(8143000538)
国家自然科学基金面上项目(8177110272)
中国博士后科学基金面上项目(2017M613351)
