摘要
目的:探讨粉防己碱(TET)对胃癌耐药细胞锌指蛋白139(ZNF139)、多耐药基因1(MDR1)及谷胱甘肽S转移酶-π(GST-π)mRNA的影响。方法:选取胃癌细胞SGC7901以及胃癌耐阿霉素细胞SGC7901/ADR,采用逆转录-聚合酶链反应和蛋白质印迹法检测两种细胞ZNF139、MDR1、GST-πmRNA及蛋白表达,同时检测2.0!g·ml-1TET处理前后SGC7901/ADR细胞ZNF139、MDR1、GST-πmRNA及蛋白表达。结果:SGC7901/ADR细胞ZNF139、MDR1、GST-πmRNA相对表达量明显高于SGC7901细胞(P<0.05);SGC7901/ADR细胞ZNF139、MDR1、GST-π蛋白相对表达量明显高于SGC7901细胞(P<0.05);TET处理后SGC7901/ADR细胞ZNF139、MDR1、GST-πmRNA及蛋白相对表达量均较处理前明显降低(P<0.05)。结论:TET能下调胃癌耐药细胞ZNF139、MDR1、GST-πmRNA和蛋白表达水平,从而在逆转胃癌多药耐药特性方面有重要作用。
Objective： To investigate the effects of tetrandrine（TET） on zinc finger protein 139（ZNF139）,multidrug resistance gene 1（MDR1） and glutathione S transferase-π（GST-π） mRNA of multidrug resistant gastric cancer cells. Methods： Gastric cancer cells SGC7901 and SGC7901/ADR were selected,and ZNF139,MDR1 and GST-π mRNA and protein expression in the two kinds of cells were detected by RT-PCR and Western blot.ZNF139,MDR1 and GST-π mRNA and protein expression of SGC7901/ADR cells were detected before and after2. 0 !g·ml-1 TET treatment. Results： ZNF139,MDR1 and GST-π mRNA relative expression of SGC7901/ADR cells were significantly higher than those of SGC7901 cells（P 〈 0. 05）; ZNF139,MDR1 and GST-π protein relative expression of SGC7901/ADR cells were significantly higher than those of SGC7901 cells（P 〈 0. 05）; After TET treatment ZNF139,MDR1 and GST-π mRNA and protein relative expression of SGC7901/ADR cells were significantly decreased compared with those before treatment（P 〈 0. 05）. Conclusion： TET can down-regulate ZNF139,MDR1 and GST-π mRNA and protein expression in drug resistant gastric cancer cells,which plays an important role in reversing multidrug resistance characteristics of gastric cancer.
作者
张旭
ZHANG Xu(Digestive Department of the First People’s Hospital of Neijiang, Neijiang 641000, Chin)
出处
《东南大学学报（医学版）》
CAS
2018年第3期467-470,共4页
Journal of Southeast University(Medical Science Edition)
关键词
胃癌
粉防己碱
锌指蛋白139
多耐药基因1
谷胱甘肽S转移酶-Π
耐药
gastric cancer
tetrandrine
zinc finger protein 139
multidrug resistance gene 1
glutathione S transferase-π
drug resistant
