摘要
为揭示消黄散作用于马火热壅盛证活性成分、作用靶点及其基因功能以及信号通路的机制,本研究采用网络药理学方法,利用中药系统药理学分析平台检索了消黄散11味中药的化学成分、作用靶点,进而构建化合物-靶点网络、蛋白质-蛋白质相互作用(PPI)网络、靶点-通路网络,研究消黄散的作用机制。结果筛选出78个化合物,相应作用靶点151个; PPI网络包含60个靶点,关键靶点主要涉及TP53、 NCOA2、 TNF、MAPK14、HSP90AB1、ESR1及VEGFA等;GO富集条目1个,分子功能1个;KEGG信号通路2条,涉及肌萎缩性脊髓侧索硬化症(ALS)通路和NOD样受体信号通路。通过对上述结果的分析,初步表明消黄散可能通过调控抑癌基因TP53、核受体共激活因子-2、肿瘤坏死因子、MAPK14蛋白等靶点,基因功能富集于类固醇绑定,ALS通路和NOD样受体信号通路以达到治疗马火热壅盛证的效果。本研究初步揭示了消黄散治疗马火热壅盛证的可能作用靶点和其作用机制,并为下一步深入探究、验证其作用机制奠定了基础。
In this study, we used the network pharmacology method to reveal the mechanism of Xiaohuangsan powder acting on horse heat syndrome with the active ingredient, target and gene function, signal pathway. Chemical components and targets related to the eleven traditional Chinese medicine （TCM） herbs were screened through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform （TCMSP）. Compound-target network, protein-protein interaction （PPI） network, and target-pathway network were constructed to explore the mechanism of Xiaohuangsan. Totally, 78 components and 151 targets related to the eleven TCM herbs were obtained. The PPI network contains 60 targets, in which key targets involving TP53,NCOA2, TNF, MAPK14, HSP90AB1, ESR1, VEGFA and so on. There were one gene ontology （GO） entries, and one steroid binding of molecular function entries. Besides, there were 2 KEGG pathways, involving Amyotrophic lateral sclerosis （ALS） and NOD-like receptor signaling pathway. In this study, we found that Xiaohuangsan regulated target genes, such as tumor suppressor gene TP53, NCOA2, TNF and MAPK14, which enriched in steroid-binding, ALS pathways and NOD-like receptor signaling pathways to treat the horse heat syndrome. Here, we preliminarily discussed the therapeutic target and mechanism of Xiaohuangsan in treating the horse heat syndrome, and those results laid the foundation for further exploration and verification of its mechanism of action.
作者
白东东
李新圃
杨峰
罗金印
王旭荣
王丹
张亚茹
李宏胜
BAI Dong-dong;LI Xin-pu;YANG Feng;LUO Jin-yin;WANG Xu-rong;WANG Dan;ZHANG Ya-ru;LI Hong-sheng(Key Laboratory of Veterinary Pharmaceutical Discovery,Ministry of Agriculture,Key Laboratory of New Animal Drug Project of Gansu Province,Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China;College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China)
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2018年第9期842-846,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
国家重点研发项目“畜禽群发普通病防控技术研究”(2017YFD0502200)
甘肃省国际科技合作项目(17YF1WA169)
中国农业科学院奶牛疾病研究创新工程项目(CAAS-ASTIP-2014-LIHPS-03)
中国农业科学院基本科研业务费专项院级统筹(Y2016PT43)
关键词
消黄散
马火热壅盛证
网络药理学
蛋白互作网络
基因本体
信号通路
Xiaohuangsan
horse heat syndrome
network pharmacology
protein-protein interaction network
gene ontdogy
signaling pathway
