摘要
目的:探讨天麻素干预对甲基苯丙胺(methamphetamine,MA)染毒大鼠血清及脑海马组织肿瘤坏死因子(TNF-α)、白细胞介素-1β(IL-1β)及脑源性神经营养因子(BDNF)含量的影响。方法:以剂量递增法建立MA染毒大鼠模型,同时分别腹腔注射天麻素(50 mg/kg、100 mg/kg)对MA染毒大鼠进行干预治疗,均连续注射10 d,第11天断头取血并分离脑海马组织,采用ELISA法检测各组大鼠血清及脑海马组织TNF-α、IL-1β、BDNF的含量。结果:与对照组比较,MA染毒模型组大鼠血清和脑海马组织TNF-α、IL-1β含量显著升高,BDNF含量显著下降(P<0.01);MA+天麻素(100 mg/kg)干预组大鼠血清、脑海马组织TNF-α、IL-1β的含量均低于MA染毒模型组(P<0.01),BDNF含量高于MA染毒模型组(P<0.01)。结论:天麻素干预可减少炎性因子含量,从而对MA引起的脑损伤有较好的保护作用。
Objective： To investigate the effects of gastrodin on the levels of TNF-α、IL-1β and BDNF in serum and hippocampus tissues of methamphetamin （MA）-exposed rats. Methods： The MA-exposed rat models were established by gradually increasing doses of MA. Gastrodin （50 mg/kg and 100 mg/kg） was intraperitoneal injected into the MA-exposed rats for 10 days. Blood samples and hippocampus tissues were taken from each rat at day 11. Then the levels of TNF-α、IL-1β and BDNF in serum and hippocampus tissues were detected by ELISA method. Results：The levels of TNF-α and IL-1β in serum and hippocampus tissues significantly increased in MA-exposed rats compared with normal rats, while the level of BDNF significantly decreased （P〈 0.01）. The levels of TNF-α and IL-1β in serum and hippocampus tissues in MA＋ gastrodin （100 mg/kg） intervention group were significantly lower than those in MA-exposed group （P〈0.01） , while the level of BDNF was significantly higher （P〈0.01）. Conclusion ： Gastrodin intervention can decrease the inflammatory factors levels of MA-dependent rats, which has protect function against MA-induced brain injury.
作者
陈晓雷
王琳
周天竹
杨秀丽
李新新
CHEN Xiaolei;WANG Lin;ZHOU Tianzhu;YANG Xiuli;LI Xinxin(Forensic Expert Testimony,Shenyang Medical College,Shenyang 110034,China;Shenyang245 Hospital)
出处
《沈阳医学院学报》
2018年第5期400-403,共4页
Journal of Shenyang Medical College
基金
辽宁省科技攻关计划项目(No.2013225086)
