摘要
为了分析链霉菌Streptomyces sp. FJS11-2菌株次级代谢产物及次级代谢产物抗菌活性筛选,采用UPLC-DAD-HRMS方法对其次级代谢产物进行组分分析.用纸片扩散法对菌株次级代谢产物进行抗菌活性测试,测试菌株为金黄色葡萄球菌、大肠杆菌、白色念珠菌、粪肠球菌及布氏杆菌. UPLC-DAD-HRMS分析结果表明,该菌株含有多种未知代谢产物.且抗菌活性研究结果表明,该菌株代谢产物具有抗金黄色葡萄球菌、布氏杆菌活性. UPLC-DAD-HRMS分析结果及抗菌活性结果,为后续从该菌株次级代谢产物中发现活性良好、结构新颖的化学成分奠定基础,同时该方法也避免化学成分重复发现.
To analyze secondary metabolites of Streptomyces sp. FJS11 - 2 and study secondary metabolites of antimicrobial activity, chemical components of secondary metabolites were analyzed by employing the UPLC - DAD - HRMS method. Antibacterial activities of secondary metabolites were tested against staphylococcus aureus, escherichia coli , Candida albicans , Enterococcus faecalis , Bacterium burgeri by employing the disc diffusion method. The UPLC - DAD - HRMS analysis results show that the strain contains a variety of unknown chemical components. The research results of antibacterial activities show that secondary metabolites of Streptomyces FJS11 - 2 have the activity against staphylococcus aureus, brinell bacillus. The results of UPLC - DAD - HRMS analysis and antimicrobial activities help lay a good foundation for the following discovery of the structurally new and active compounds from secondary metabolites of Streptomyces sp. FJS11 -2. At the same time, the method also avoids a repeated f/nding of the same chemical compositions.
作者
李彩玉
钱声艳
秦立达
LI Cai-yu;QIAN Sheng-yan;QIN Li-da(Special Key Laboratory of Oral Disease Research,Higher Education Institution in Guizhou Province,Zunyi Medical University,Zunyi 563000,China;Special Key Laboratory of Microbial Resources and Drug Development,Higher Education Institution in Guizhou Province,Zunyi Medical University,Zunyi 563000,China;Child Rehabilitation Section,Liaocheng Maternal and Child Health Hospital,Liaocheng 252000,China)
出处
《云南民族大学学报（自然科学版）》
CAS
2018年第5期372-376,共5页
Journal of Yunnan Minzu University:Natural Sciences Edition
基金
遵义市科技局市校联合基金(E-215)
贵州省普通高等学校微生物资源与药物开发特色重点实验室开放课题基金(GZMRD-2015-002
GZMRD-2015-003)
