摘要
大豆基因工程育种和功能基因组分析亟需建立高效大豆转化体系。本文综述了近年来影响农杆菌介导大豆遗传转化体系的农杆菌因素、植物因素及培养条件等方面的进展,主要包括农杆菌菌株、菌株生长状态、大豆基因型、外植体类型、防御酶、内源激素及侵染和共培养方法等,为提高大豆遗传转化效率及基因工程育种等相关研究提供参考。
There is an urgent need to establish a high-efficiency transformation system for soybean genetic engineering breed-ing and functional genome analysis. This article summarized research advances of affecting factors including Agrobacterium fac-tors, plants factors and culture conditions that affect Agrobacterium tumefaciens mediated transformation rencent years, mainly a-bout Agrobacterium strains, bacterial growth state, soybean genotypes, explant types, defense enzymes, endogenous hormones and the condition of infection and co-cuhure. It will would provide reference for correlational studies of improving soybean transformation efficiency and genetic engineering breeding.
作者
杨柳
于翠梅
刘铭
赵明哲
谢甫绨
YANG Liu;YU Cui-mei;LIU Ming;ZHAO Ming-zhe;XIE Fu-ti(Agronomy college of Shenyang Agroeultural University, Shenyang 110866, China)
出处
《大豆科学》
CAS
CSCD
北大核心
2018年第5期803-808,共6页
Soybean Science
基金
辽宁省教育厅项目(L2015490)
辽宁省科技厅项目(2015020762)
关键词
大豆
农杆菌
转化
影响因素
Soybean
Agrobacterium
Transformation
Affecting factors
