摘要
为建立一个大豆子叶节高效遗传转化体系用于大豆基因工程育种,以超高产大豆品种为材料,子叶节为外植体进行了遗传转化,研究了农杆菌侵染的菌液浓度、侵染时间、共培养基中乙酰丁香酮浓度及共培养时间等影响农杆菌转化的因素。结果表明:适宜转化的侵染和共培养条件为侵染菌液浓度OD600=0.5,侵染时间30 min,共培养基含有乙酰丁香酮浓度200μmol·L^-1,共培养时间为3-4 d。并以此条件对不同基因型超高产大豆品种进行农杆菌转化,结果显示大豆基因型对农杆菌的敏感性存在显著性差异,以沈农9号最敏感,沈农12次之,中黄35最差。
To establish a high frequency genetic transformation system of soybean cotyledon node for soybean genetic engineering,Agrobacterium concentration,infection time,co-cultivation time and acetosyringone concentration of co-culture medium during soybean cotyledonary node transformation were studied by using soybean cultivars with super-high-yielding. The results showed that the optimal conditions were Agrobacterium concentration OD600= 0. 5,infection time 30 min,co-cultivation time 3-4days and acetosyringone concentration 200 μmol·L-1. Using the optimized transformation procedure,different genotypes of super-high-yielding soybeans were transformed and the sensitivity of soybean genotypes to Agrobacterium tumefaciens was significant differently. Shennong 9 was the most sensitive material,followed by Shennong 12,and Zhonghuang 35 was the least sensitive material.
出处
《大豆科学》
CAS
CSCD
北大核心
2014年第5期634-637,共4页
Soybean Science
基金
转基因生物新品种培育重大专项(2013ZX08004-004-003-004)
关键词
超高产大豆
根癌农杆菌
子叶节
转化
Super-high-yielding soybeans
Agrobacterium tumefaciens
Cotyledonary node
Transformation