摘要
文章采用子叶节法,利用高再生率大豆品种合丰25与低再生率大豆品系L-28及其衍生的重组自交系群体(RIL)100个家系,评价不定芽诱导率、伸长率、生根率和成苗率等指标,比较不同大豆基因型之间再生率差异,并利用简化基因组测序技术作QTL定位分析。结果表明,在大豆RIL群体4个再生性状中,诱导率与伸长率、生根率和成苗率之间均达极显著,表现为连续单峰曲线,符合正态分布特点。同时检测到10个与大豆再生性状显著相关QTL位点,其中独立QTL位点2个,可重复检测QTL位点3个,与诱导率、伸长率、生根率和成苗率显著相关QTL位点分别为2个、3个、3个、2个。研究结果将为探索大豆再生规律、建立高效再生体系、提高大豆遗传转化效率奠定基础。
Using the cotyledon node method and recombinant inbred line population(RIL) 100 families derived from high regeneration rate soybean variety Hefeng 25 and low regeneration rate soybean line L-28, adventitious shoot induction rate, elongation rate, rooting rate and seedling rate were evaluated in order to compare the regeneration rate differences among different soybean genotypes,and simplified genome sequencing technology was used for QTL mapping analysis. The results showed that among the four regeneration traits of soybean RIL population, the induction rate and elongation rate, rooting rate and seedling rate all reached extremely significant difference levels, and all showed continuous unimodal curves, conforming to a normal distribution. Total 10 QTL loci which significantly related to soybean regeneration traits were detected, including two independent QTL loci and three repeatable QTL loci. QTL loci which were significantly related to induction rate, elongation rate, rooting rate and seedling rate were 2, 3, 3, and 2, respectively. The results could lay the foundation for exploring soybean regeneration rules, establishing an efficient regeneration system, and improving soybean genetic transformation efficiency.
作者
滕卫丽
郑立娜
张琦
赵雪
韩英鹏
李文滨
TENG Weili;ZHENG Lina;ZHANG Qi;ZHAO Xue;HAN Yingpeng;LI Wenbin(Institute of Soybean Research,Key Laboratory of Soybean Biology in Chinese of Ministry Education,Key Laboratory of Soybean Biology and Breeding(Genetics)of Chinese Agriculture and Rural Ministry,Northeast Agricultural University,Harbin 150030,China)
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2021年第4期1-10,共10页
Journal of Northeast Agricultural University
基金
转基因生物新品种培育重大专项(2016ZX08004001-007)
现代农业产业技术体系建设专项资金项目(CARS-04-PS04)
黑龙江省“百千万”工程科技重大专项课题(2019ZX16B01-1)。
关键词
大豆
再生体系
QTL
soybean
regeneration system
QTL
