摘要
的 研究心房利钠肽（atrial natriuretic peptide,ANP）对毛细支气管炎（简称毛支）模型小鼠肺组织辅助性T细胞（helper T cell,Th）17细胞分化的影响,为毛支治疗提供理论基础.方法 按照随机对照原则将ALB/C小鼠分为正常组、呼吸道合胞病毒（respiratory syncytial virus,RSV）组、ANP＋ANP抑制剂（A71915）组和ANP组,滴鼻法成功建立毛支模型,而后雾化吸入ANP或腹腔注射A71915,收集支气管肺泡灌洗液（bronchoalveolar lavage fluid,BALF）检测白细胞介素（interleukin,IL）-17含量,留取肺组织进行HE染色、免疫组织化学染色和Western blot检测,观察RORγt的蛋白表达及气道炎症情况.结果 与正常组相比,RSV组小鼠IL-17及RORγt的含量增高（P〈0.05）;与RSV组相比,ANP组小鼠IL-17及RORγt的表达量均降低（P〈0.05）,ANP＋A71915组各项指标较RSV组相比无显著差异（P〉0.05）.结论 ANP能够抑制毛支小鼠Th17细胞的分化.
Objective To investigate the effects of eatrial natriuretic petide（ANP）on the differentia-tion of Th17 cells in the lung tissue of bronchiolitis mice and provide a theoretical basis for the treatment of bronchiolitis. Methods BALB/ C mice were divided into normal group,bronchiolitis group（RSV）,ANP ＋A71915 group and ANP group,The bronchiolitis model was successfully established by intranasal nasal drip-ping,and then treated with ANP or A71915 to collect bronchoalveolar lavage fluid（BALF）to detect the level of IL-17,the lung tissue was taken for RORγt and airway inflammation by HE staining,immunohistochemical stai-ning and Western blot. Results Compared with the normal group,the levels of IL-17 and RORγt in the RSV group were significantly increased. Compared with the RSV group,the expression of them in the ANP group was significantly decreased （P 〈 0. 05）. There was no significant difference between ANP ＋ A 71915 group and RSV group（P 〉 0. 05）. Conclusion ANP can inhibit the differentiation of Th17 cells in bronchiolitis mice.
作者
张雪静
何潇
吴福玲
刘良宵
高萌
Zhang Xuejing;He Xiao;Wu Fuling;Liu Liangxiao;Gao Meng(Department of Pediatrics,Affiliated Hospital of Binzhou Medical University,Binzhou 256600,China)
出处
《国际免疫学杂志》
CAS
2018年第4期384-389,共6页
International Journal of Immunology
基金
山东省自然科学基金（ZR2014HL001）
山东省滨州市软科学研究计划项目（2016BRK19）
