摘要
目的探讨鼻咽癌组织中微小RNA-328(mi R-328)的表达及其对鼻咽癌CNE-2细胞侵袭和迁移的影响。方法采用实时荧光定量PCR(QPCR)检测86例鼻咽癌组织和15例慢性鼻咽炎组织及鼻咽癌细胞系CNE-2、HK1和正常鼻咽上皮细胞NP69中的mi R-328水平,分析mi R-328水平与鼻咽癌临床病理特征(年龄、性别、临床分期、淋巴结转移、复发和生存状态)的关系;采用脂质体Lipofectamine 2000法向对数生长期CNE-2细胞转染mi R-328模拟物(mimics)或阴性对照(NC),采用QPCR检测转染48 h后的mi R-328水平以评价转染效率,采用Transwell迁移和侵袭实验检测转染后CNE-2细胞的穿膜细胞数目。结果 QPCR检测结果显示,鼻咽癌细胞CNE-2、HK1中mi R-328水平低于正常鼻咽上皮细胞株NP69(P<0. 05);86例鼻咽癌组织的mi R-328水平亦低于15例慢性鼻咽炎组织,差异有统计学意义(P<0. 05)。mi R-328水平与鼻咽癌患者的复发状态(P=0. 037)、临床分期(P=0. 005)和生存状态(P=0. 012)有关,但与年龄、性别和淋巴结转移无关(P>0. 05)。转染mimics 48 h后的细胞中mi R-328水平大幅升高,与转染NC的细胞比较,差异有统计学意义(P<0. 05)。Transwell实验结果显示,转染mi R-328 mimics后,迁移和侵袭实验中CNE-2细胞的穿膜数目均降低,与转染NC的细胞比较,差异有统计学意义(P<0. 05)。结论 mi R-328在鼻咽癌组织和细胞中低表达,且参与鼻咽癌的发生发展,上调其水平可抑制迁移侵袭过程,在鼻咽癌防治中有一定价值。
Objective To investigate the expression of microRNA-328 （miR-328） in nasopharyngeal carcinoma tissues and its effect on invasion and migration of CNE-2 cells.Methods The quantitative real-time PCR （QPCR） was used to detect the levels of miR-328 in 86 cases of nasopharyngeal carcinoma tissues and 15 cases of chronic nasopharyngitis as well as nasopharyngeal carcinoma cell lines CNE-2, HK1 and normal nasopharyngeal epithelial cell line NP69. The relationship between the levels of miR-328 and the clinicopathological features （age, sex, clinical stage, lymph node metastasis, recurrence and survival status） of nasopharyngeal carcinoma was analyzed. MiR-328 mimics or negative control （NC） were transfected into CNE-2 cells at logarithmic phase by liposome Lipofectamine 2000. The miR-328 level was detected by QPCR at 48 h after transfection to evaluate the transfection efficiency. The numbers of penetrating cells of CNE-2 cells were detected by Transwell migration and invasion assay.Results The results of QPCR showed that the miR-328 levels in nasopharyngeal carcinoma cell lines CNE-2 and HK1 were lower than those in normal nasopharyngeal epithelial cell line NP69 （ P 〈0.05）, and the miR-328 levels in 86 cases of nasopharyngeal carcinoma tissues were lower than those in 15 cases of chronic nasopharyngitis （ P 〈0.05）. The miR-328 level was related to the recurrence （ P =0.037）, clinical stage （ P =0.005） and survival （ P =0.012）, but not to age, sex and lymph node metastasis （ P 〉0.05）. After 48-h transfection, the miR-328 level in cells transfected with mimics was significantly higher than that in cells transfected with NC （ P 〈0.05）. Transwell assay showed that the numbers of CNE-2 cells transfected with mimics were significantly lower than those with NC （ P 〈0.05）.Conclusion miR-328 is lowly expressed in both nasopharyngeal carcinoma tissues and cells, and participates in the occurrence and development of nasopharyngeal carcinoma. Up-regulating its level can
作者
汪祖益
刘及江
WANG Zuyi;LIU Jijiang(Department of Otolaryngology Head and Neck Surgery,Chongqing Kaizhou District People s Hospital,Chongqing 405400,China)
出处
《临床肿瘤学杂志》
CAS
北大核心
2018年第9期806-810,共5页
Chinese Clinical Oncology
关键词
鼻咽癌
微小RNA-328
迁移侵袭
Nasopharyngeal carcinoma
MicroRNA-328
Migration and invasion
