摘要
目的观察风湿定胶囊对类风湿性关节炎(RA)大鼠滑膜组织中JAK-STAT信号通路的影响。方法取85只大鼠,随机选出10只为空白组(予等量生理盐水),其余75只注射Ⅱ型胶原制备RA大鼠模型;将造模成功大鼠随机分为模型组(等量生理盐水)、阳性对照组[甲氨蝶呤0.5 mg/(kg·d)]、风湿定胶囊低剂量组[200 mg/(kg·d)]、中剂量组[400 mg/(kg·d)]、高剂量组[800 mg/(kg·d)];观察并记录给药后不同时间段大鼠体质量变化等一般情况及右后足肿胀体积;给药4周后,以免疫组化法观察各组大鼠滑膜组织病理变化,Western blotting测定各组大鼠关节滑膜组织中p-JAK3、p-STAT3的蛋白表达水平。结果模型组关节肿胀明显,摄食及活动减少,精神萎靡;给药治疗后,各治疗组大鼠摄食及精神状态较模型组明显改善,且随治疗时间延长,效果越明显;模型组大鼠体质量增加低于治疗组。治疗4周后,模型组及各治疗组大鼠足肿胀体积均显著高于空白组;随风湿定胶囊剂量的增加,肿胀体积逐渐减小(P<0.05),且中、高剂量组效果显著优于阳性对照组(P<0.05)。各组间JAK3蛋白及STAT3蛋白表达水平差异无统计学意义(P>0.05);模型组及各治疗组大鼠关节滑膜组织中p-JAK3、p-STAT3的蛋白表达水平均显著高于空白组(P<0.05);随风湿定胶囊剂量的增加,p-JAK3、p-STAT3的蛋白表达水平均逐渐下调(P<0.05),且中、高剂量组下调效果显著优于阳性对照组(P<0.05)。结论风湿定胶囊可能通过下调RA大鼠关节滑膜组织中p-JAK3、p-STAT3的表达调控JAK/STAT信号通路进行RA的治疗。
Objective： To observe the influence of Fengshiding Capsule on JAK/STAT signal pathway in synovial tissue of Rats with rheumatoid arthritis（RA）. Methods： 85 rats were selected,10 ones as the blank control group（ig the normal saline）,and the other 75 rats were injected type Ⅱ collagen as RA rat model. Successfully modeled rats were divided into the model group（ig the normal saline）,the positive control group[ig 0.5 mg/（kg·d）methotrexate],Fengshiding Capsule low dose group [ig 200 mg/（kg·d）],middle dose group [ig 400 mg/（kg·d）]and high dose group [ig 800 mg/（kg·d）]. General changes of body weight in rats at different time periods and swelling volume of right hind foot were observed and recorded after administration,and after administration for 4 weeks,pathological changes of synovial tissue in rats of each group were observed with immunohistochemical method,and the expression levels of p-JAK3 and p-STAT3 in synovial tissue of rats in each group were detected with Western blotting. Results： Joint swelling of the model group was obvious;the feeding and activities decreased,and the mental disorder occurred. After treatment,the feeding and mental state of the rats in each treatment group significantly improved,compared with those in the model group,and the effect was more obvious with the extension of treatment time. Weight gain in the model group was lower than those in the treatment groups. Af-ter 4 weeks′ treatment,the volumes of foot swelling in the model group and the treatment groups were significantly higher than that in the blank group. With the dose increase of Fengshiding Capsule,swelling volume decreased gradually（P〈0.05）,and the effects of the middle and high dose groups were significantly better than that in the positive control group（P 〈0.05）. There was no significant difference in JAK3 and STAT3 protein expression levels among the groups（P〉0.05）,and p-JAK3 and p-STAT3 expression levels in synovial tissue of the model group and the treatment group
作者
陈勇
陈涛
高永翔
Chen Yong;Chen Tao;Gao Yongxiang(The Affiliated Hospital of Chengdu University of Tradi-tional Chinese Medicine,Sichuan,Chengdu 610000,China.)
出处
《中国中医急症》
2018年第9期1522-1526,共5页
Journal of Emergency in Traditional Chinese Medicine
基金
四川省卫生厅科研基金项目(150051)
