摘要
目的观察佐剂性关节炎(AA)大鼠外周血血小板微颗粒(PMPs)及血小板参数、滑膜组织血管内皮生长因子(VEGF)、内皮抑素(ES)、微血管密度(MVD)的表达。方法将24只SD大鼠随机数字表法分为健康对照(NC)组、模型对照(MC)组,通过注射弗氏完全佐剂复制AA模型。造模成功30 d后,观察两组大鼠足趾肿胀度(E)、关节炎指数(AI)、关节病理损伤指数(JPI)、血小板参数、PMPs及滑膜ES、VEGF、MVD的变化。结果与NC组比较,MC组E、AI、JPI、MVD、血小板参数(PLT、PCT)、PMPs及滑膜VEGF mRNA表达升高,滑膜ES mRNA表达降低,VEGF mRNA/ES mRNA升高。相关性分析结果表明PMPs与血小板参数PLT、PCT呈正相关; PMPs与AI、E、JPI、MVD、VEGF mRNA、VEGF mRNA/ES mRNA呈正相关; PLT与E、MVD呈正相关; PCT与E、AI呈正相关(P <0. 05)。结论 AA大鼠PMPs表达升高可能是引起VEGF/ES表达失衡,导致滑膜组织血管增生、关节损伤的原因。
Objective To observe the expression of PMPs, platelet parameters,VEGF, ES, MVD in the adjuvant arthritis. Methods 24 SD rats were randomly divided into normal control（NC） group and model control（MC） group. AA model was made by injecting freundg complete adjuvant. AFter modeling, to observe two groups of E, AI, Pathological injury- index of synovial joint,PMPs, platelet parameters, VEGF, ES, MVD. Results Compared with NC group, the ex- pression of E, AI, synovial joint pathological damage index, MVD （ PLT, PCT） , platelet parameters, PMPs and synovial VEGF mRNA,VEGF mRNA/ES mRNA of the MC group were rising, synovial ES mRNA expression of the MC group was reducing. PMPs was positively correlated with PLT, PCT. PMPs were positively correlated with AI, E, JPI, MVD, VEGF mRNA, VEGF mRNA/ ES mRNA. PLT was positively correlated with E and MVD. PCT was positively correlated with E and AI （ P 〈 0.05 ）. Conclusion The increase of PMPs in AA rats may be the cause of vascular hyperplasia and joint injury- of synovial tissue caused by the imbalance of VEGF/ES.
作者
刘磊
刘健
黄传兵
万磊
谌曦
Liu Lei;Liu Jian;Huang Chuanbing;Wan Lei;Shen Xi(Department of Rheumatology,the First Affiliated Hospital,Anhui University of Chi-nese Medicine,Hejbi 230031,China)
出处
《中国临床保健杂志》
CAS
2018年第5期656-660,共5页
Chinese Journal of Clinical Healthcare
基金
国家自然科学基金(81473672)
2016年中央引导地方科技发展专项(财教[2016]1188)
安徽省科技攻关项目(1604a0802085)
现代中医内科应用基础与开发研究安徽省重点实验室(财教[2016]518)
2015年技术创新服务体系专项(财教[2015]1551)
国家临床重点专科中医风湿病学建设项目(财社[2013]239号)
关键词
关节炎
实验性
血小板
滑膜
疾病模型
动物
Arthritis
experimental
Blood platelets
Synovial membrane
Disease models
animal
