摘要
目的探讨大蒜素对人黑素细胞氧化应激损伤的保护作用及机制。方法设空白组、对照组、过氧化氢组、大蒜素组、实验1、2、3组。空白组无细胞仅添加完全培养基；对照组添加完全培养基；过氧化氢组添加0.4 mmol/L过氧化氢完全培养基；大蒜素组添加新鲜配制的浓度为40 μmol/L大蒜素完全培养基；实验1、2、3组，首先分别以不同浓度大蒜素（80、40、20 μmol /L大蒜素完全培养基）干预对数生长期经0.4 mmol /L过氧化氢处理的黑素细胞，药物干预24 h后用倒置显微镜观察细胞形态，采用噻唑蓝（MTT）比色法测定大蒜素对氧化应激损伤黑素细胞的保护作用。结果不同浓度大蒜素对过氧化氢诱导下黑素细胞保护作用不同。过氧化氢组黑素细胞活力显著下降（43.610±3.872） %（P〈0.05），但与实验2、3组相比差异无统计学意义（P＝0.345）；实验1组黑素细胞活力明显降低（58.223±2.806）% （P〈0.05），但高于实验2、3组（P〈0.05）。结论大蒜素通过降低H2O2诱导下人黑素细胞胞内活性氧自由基（ROS）产生，调控人黑素细胞氧化应激水平，抵抗H2O2引起的细胞凋亡。大蒜素可能是机体介导氧化应激反应的保护性因素。
ObjectiveTo investigate the protective effect and mechanism of garlicin on oxidative stress injury of human melanocytes.MethodsThere were blank group, control group, hydrogen peroxide group, garlicin group, experiment 1, 2 and 3 groups. No cells in the blank group were only added with complete culture medium. The control group was added with complete medium; 0.4 mmol/L hydrogen peroxide complete medium was added to the hydrogen peroxide group. Garlicin group was added with freshly prepared garlicin complete culture medium with concentration of 40 μmol/L; Experiment groups 1, 2, and 3 were treated with different concentrations of garlicin （80, 40, and 20 μmol/L garlicin complete medium, respectively） to interfere with melanocytes treated with 0.4 mmol/L hydrogen peroxide during logarithmic growth period. After 24 h of drug intervention, the cell morphology was observed under an inverted microscope. The protective effect of garlicin on melanocytes damaged by oxidative stress was measured by MTT colorimetric method.ResultsDifferent concentrations of garlicin had different protective effects on melanocytes induced by hydrogen peroxide. It could be concluded that the activity of melanocytes in hydrogen peroxide group decreased significantly （43.610 ± 3.872）% （P〈0.05）, but there was no statistical difference between the two groups （P=0.345）. The activity of melanocytes in experimental group 1 was significantly decreased （58.223 ± 2.806）% but higher than that in experimental groups 2 and 3 （P〈0.05）.ConclusionsAllicin inhibits the production of intracellular ROS in human melanocytes induced by H2O2, regulates oxidative stress in human melanocytes, and counteracts H2O2-induced apoptosis. Therefore, allicin may be a protective factor in mediating oxidative stress in the body.
作者
唐亚平
柯娅楠
刘玉梅
李振洁
李仰琪
王建琴
Tang Yaping;Ke Yanan;Liu Yumei;Li Zhenjie;Li Yangqi;Wang Jianqin(Guangzhou Institute of Dermatology,Guangzhou 510095,China)
出处
《中华医学美学美容杂志》
2018年第4期282-285,共4页
Chinese Journal of Medical Aesthetics and Cosmetology
基金
广东省医学科研基金项目（A2018288）
