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大黄素对人表皮黑素细胞增殖、黏附、迁移、c-kit mRNA及蛋白表达的影响

Effects of emodin on proliferation, adhesion, migration and c-kit mRNA and protein expression of human epidermal melanocytes
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摘要 目的探讨大黄素对人表皮黑素细胞增殖、黏附、迁移、c-kit mRNA及蛋白表达的影响。方法2022年5月至2023年2月,前海人寿广州总医院皮肤科用不同浓度的大黄素干预体外培养的人表皮黑素细胞,实验设空白对照组(含培养基+人表皮黑素细胞)和实验组(含培养基+人表皮黑素细胞+不同浓度大黄素)。用CCK-8法检测细胞增殖活力,微孔板法检测细胞黏附能力,Transwell微孔膜法检测细胞迁移能力,反转录-聚合酶链反应法检测细胞c-kit mRNA表达水平,Western blot蛋白印迹法检测细胞c-kit蛋白表达水平。结果与空白对照组相比,实验组大黄素降低了人表皮黑素细胞增殖、黏附及迁移活力,差异有统计学意义(F=391.48,P<0.0001;F=10.93,P=0.003;F=7.75,P=0.009)。与空白对照组相比,实验组大黄素对人表皮黑素细胞c-kit mRNA及蛋白的表达具有双向作用,高浓度大黄素抑制c-kit mRNA及蛋白的表达,低浓度大黄素促进c-kit mRNA及蛋白的表达,差异有统计学意义(F=11.49,P=0.003;F=2155.11,P<0.001)。结论高浓度大黄素抑制c-kit mRNA及蛋白的表达,低浓度大黄素促进c-kit mRNA及蛋白的表达,为大黄素治疗色素性皮肤病提供了依据。 Objective To investigate the effects of emodin on proliferation,adhesion,migration,c-kit mRNA and protein expression of human epidermal melanocytes.Methods Human epidermal melanocytes cultured in vitro were treated with different concentrations of emodin,a blank control group(containing medium+human epidermal melanocytes)and an experimental group(containing medium+human epidermal melanocytes+different concentrations of emodin)were set up.Cell proliferation was measured by CCK-8 method,cell adhesion was measured by microplate assay,cell migration was measured by Transwell membrane assay,and c-kit mRNA expression was measured by reverse transcription-polymerase polymerase chain reaction;Western blot was used to detect the expression of c-kit protein.Results Compared with the blank control group,emodin decreased the proliferation,adhesion and migration of human epidermal melanocytes,the difference was statistically significant(F=391.48,P<0.0001;F=10.93,P=0.003;F=7.75,P=0.009).Compared with the blank control group,emodin in the experimental group had a bidirectional effect on the expression of c-kit mRNA and protein.High concentration of emodin inhibited the expression of c-kit mRNA and protein,and the expression of c-kit mRNA and protein was significantly increased by Emodin(F=11.491,P=0.003;F=2155.11,P<0.001).Conclusions Emodin inhibits the proliferation,adhesion and migration of human epidermal melanocytes,high concentration of emodin inhibits the expression of c-kit mRNA and protein,and low concentration of emodin promotes the expression of c-kit mRNA and protein,and the results provide a basis for the clinical application of emodin in pigmented dermatosis.
作者 任英云 李其林 李嘉萍 吴章武 Ren Yingyun;Li Qilin;Li Jiaping;Wu Zhangwu(Department of Dermatology,Foresea Life Insurance Guangzhou General Hospital,Guangzhou 511300,China;Department of Dermatology,Guangzhou Red Cross Hospital Affiliated to Jinan University,Guangzhou 510220,China)
出处 《中华医学美学美容杂志》 2023年第6期439-443,共5页 Chinese Journal of Medical Aesthetics and Cosmetology
基金 广州市中医药和中西医结合科技项目 (20222A010089)。
关键词 黑素细胞 大黄素 细胞增殖 细胞黏附 细胞迁移 C-KIT Melanocytes Emodin Cell proliferation Cell adhesion Cell migration C-kit
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