摘要
目的观察＂嗅三针＂干预脂多糖（LPS）经鼻诱导的帕金森病小鼠行为学、嗅球病理改变以及酪氨酸羟化酶（TH）在黑质中的表达,阐明针刺＂嗅三针＂对帕金森病早期的干预效应是通过改善大鼠嗅觉障碍而发挥作用。方法取C57BL/6雄性小鼠40只,随机分为空白组（Sham）,帕金森模型组（Model）,帕金森模型＋嗅三针干预组（XSZ）,左旋多巴组（L-DOPA）,每组10只。除空白组外,其余各组均用经鼻灌注LPS的方法建立帕金森模型,造模同时进行电针干预10 d,用HE染色法观察嗅球病理变化以及免疫组织化学法测定组织中TH的表达水平。结果嗅三针有保护帕金森模型嗅球病理改变的作用,也可以显著提高黑质TH的表达水平（P〈0.05）,与左旋多巴组疗效相当。结论 ＂嗅三针＂干预可以改善LPS诱导的小鼠帕金森病行为学及病理学改变,其机制可能与降低TH在小鼠大脑黑质的表达有关。
Objective To observe the effect of Xiusanzhen on the behavior, pathological changes of olfactory bulb and the expression of tyrosine hydroxylase（TH） in substantia nigra induced by lipopolysaccharide（LPS） in mice with Parkinson＇s disease. The purpose of this study is to elucidate that the intervention effect of acupuncture Xiusanzhen on Parkinson＇s disease in the early stage may play a role by improving olfactory disorders in rats. Methods 40 C57 BL/6 male mice were randomly divided into blank group（Sham）, Parkinson＇s model group（Model）, Parkinson＇s model and Xiusanzhen intervention group（XSZ）, L-DOPA group（10 rats in each group）. In addition to the blank group, the other groups were established by nasal perfusion LPS model, modeling and electroacupuncture intervention for 10 days at the same time, the pathological changes of olfactory bulb and the expression of TH in tissues were observed by HE staining and immunohistochemistry. Results Xiusanzhen could protect the pathological changes of olfactory bulb in Parkinson＇s model and also significantly increase the expression of TH in substantia nigra（P〈0.05）, which was equivalent to that in levodopa group. Conclusion Xiusanzhen intervention can improve the behavioral and pathological changes of Parkinson＇s disease induced by LPS in mice, and the mechanism might be related to the decrease of TH expression in the substantia nigra of mice brain.
作者
王强
刘智斌
王渊
李杰
鲁刚
马雪
郭阳
WANG Qiang;LIU Zhibin;WANG Yuan;LI Jie;LU Gang;MA Xue;GUO Yang(Shaanxi University of Chinese Medicine,Xianyang 712046,Shaanxi Province,China)
出处
《长春中医药大学学报》
2018年第4期634-637,共4页
Journal of Changchun University of Chinese Medicine
基金
国家自然科学基金(81674086)
关键词
帕金森病
嗅三针
嗅觉障碍
酪氨酸羟化酶
Parkinson＇s disease
Xiusanzhen
olfactory disorder
Tyrosine hydroxylase
