摘要
目的:探讨电针治疗帕金森病(PD)的作用机制。方法:将50只Wistar大鼠随机分为正常组、假手术组、模型组、风府太冲组和双固一通组,以6-羟基多巴胺右侧纹状体微量注射法制备偏侧PD大鼠旋转模型,假手术组以生理盐水替代6-羟基多巴胺进行微量注射。正常组、假手术组、模型组不做任何治疗;风府太冲组在造模的基础上电针"风府""太冲"穴,双固一通组在造模基础上电针"风府""太冲""关元""足三里"4穴,两组均每日治疗1次,共治疗2周。对比观察针刺"风府""太冲"和"双固一通"取穴法对PD模型大鼠胶质细胞源性神经营养因子(GDNF)及其功能性受体Ret表达的影响。采用免疫组化法检测GDNF阳性细胞数量,以免疫印迹法检测Ret含量。结果:两电针组GDNF阳性细胞数量比其他组显著增加(均P<0.01),双固一通组作用强于风府太冲组(P<0.01);两电针组Ret含量亦显著高于其他组(均P<0.01)。结论:电针治疗能使PD大鼠黑质和纹状体GDNF表达增强,使黑质Ret高表达,说明电针不仅能提高GDNF的表达水平,还能提高GDNF的作用效应,"双固一通"取穴在提高GDNF表达方面较单纯取"风府""太冲"穴作用强。
Objective To explore the mechanism of electroacupuncture therapy on Parkinson＇s disease （PD）. Methods Fifty Wistar rats were randomly divided into a normal group, a sham-operation group, a model group, a Fengfu-Taichong group and a Shuanggu Yitong group. PD model was duplicated by microinjection of 6-Hydroxyl- Dopamine into right corpora striata, and by microinjection of normal saline in sham-operation group. Rats in normal group, sham-operation group and model group were not treated. In Fengfu-Taichong group, the rats were treated by electroacupuncture at ＂Fengfu＂ （GV 16） and ＂Taichong＂ （LR 3） on the basis of the PD model, and by electro- acupuncture at ＂Fengfu＂ （GV 16）, ＂Taichong＂ （LR 3）, ＂Guanyuan＂ （CV 4） and ＂Zusanli＂ （ST 36） in Shuanggu Yitong group, once daily for 2 weeks. GDNF and Ret expression were detected by immunohistochemistry and western blotting, respectively. Results The number of GDNF positive cells and the content of Ret receptor increased significantly in the two electroacupuncture groups compared with those in the other groups （all P〈0.01）, and the expression of GDNF increased significantly in Shuanggu Yitong group compared with that in Fengfu-Taichong group （P〈0.01）. Conclusion Electroacupuncture can not only increase the expression of GDNF, but also enhance its effect. ＂Shuanggu Yitong＂ method is better than simple acupuncture at ＂Fengfu＂ （GV 16） and ＂Taichong＂ （LR 3） in increasing expression of GDNF.
出处
《中国针灸》
CAS
CSCD
北大核心
2010年第9期739-743,共5页
Chinese Acupuncture & Moxibustion
基金
国家自然科学30300461
30973787
30973809
湖北省教育厅科学技术研究项目:2002B00004
D200726001
Z20091601
湖北省科技厅科学技术项目:2005AA301C32
2009CDA068
武汉市科学技术局科学技术研究项目:20036004056
201060623269
关键词
帕金森病
电针
胶质细胞源性神经营养因子
Parkinson＇s Disease
Electroaeupuneture
Glial Cell Line-Derived Neurotrophic Factor
