摘要
本文研究了泥沙悬浮液浊度对叶绿素荧光计测定水样中活体叶绿素荧光的影响。结果表明,浊度产生的荧光效应和遮蔽效应对活体叶绿素荧光测定具有显著影响。在100NTU浊度范围内,浊度会导致荧光效应使活体叶绿素荧光值测量结果随着浊度的增大而增大,并构成良好的线性关系;实际测定水体中硅藻的活体叶绿素荧光值时,泥沙悬浮液的浊度会导致遮蔽效应使活体叶绿素荧光值测量结果随着浊度的增大而减小,水体中硅藻质量浓度越高遮蔽效应越显著,在1 000 mV(约10μg/L)硅藻质量浓度,100NTU浊度条件下,浊度遮蔽效应可导致活体叶绿素荧光值偏低20%。根据近海泥沙实验数据,本文提出了一种活体叶绿素荧光检测的浊度影响修正方法,在100NTU浊度内,修正后叶绿素荧光值偏差率都下降到±10%以内,校准后的荧光值与真实叶绿素荧光值较为接近。
The influence of sediment turbidity on chlorophyll sensor determination of in vivo chlorophyll fluorescence was studied.The results show that turbidity can cause fluorescence effect and light shielding effect on the determination of chlorophyll fluorescence.In the range of 100 NTU turbidity,the fluorescence value of chlorophyll shows a good linear relationship with the turbidity of sediment suspension,which increases with the increase of turbidity.When actually measuring the in vivo chlorophyll fluorescence of diatoms in water,turbidity of sediment suspension causes light shielding effect that decreases in vivo chlorophyll fluorescence as turbidity increases.The concentration of microalgae in the water is higher,the light shielding effect is stronger.In 1 000 mV（including 10μg/L）,the highest concentration of microalgae,sediment suspension can reduce the chlorophyll fluorescence value by 20% at 100 NTU turbidity.Based on the experimental data,a correction method of turbidity effect of in vivo chlorophyll fluorescence detection is proposed.Within 100 NTU turbidity,the corrected deviation of chlorophyll fluorescence values is reduced to less than ±10% and the corrected fluorescence value is close to the true chlorophyll fluorescence.
作者
叶陈军
潘建明
郑旻辉
谢尚微
章哲超
YE Chen-jun;PAN Jian-ming;ZHENG Min-hui;XIE Shang-wei;ZHANG Zhe-chao(Laboratory of Marine Ecosystem and Biogeochemistry,SOA,Hangzhou 310012,China;Second Institute of Oceanography,SOA,Hangzhou 310012,China)
出处
《海洋学研究》
CSCD
北大核心
2018年第2期27-35,共9页
Journal of Marine Sciences
基金
浙江省自然科学基金项目资助(LQ16D060006)
中央级公益性科研院所基本科研业务费专项项目资助(JG1715
JT1502)
国家海洋公益性行业科研专项项目资助(201305012)
关键词
荧光计
叶绿素荧光
浊度
泥沙
修正方法
Fluorometer
chlorophyll fluorescence
turbidity
sediment
correction method
