摘要
建立了一种反相高效液相色谱法(RP-HPLC)测定浒苔(Enteromorpha prolifera)中叶绿素a和b含量。采用的色谱条件:Diamonsil-C18(4.6mm×150mm)柱,流动相为甲醇(含0.2%的乙酸)和丙酮,梯度洗脱,流速为0.8mL/min,进样量10μL,检测波长430nm。在此条件下,叶绿素a和叶绿素b与浒苔中的其他化合物分离良好,叶绿素a、b质量浓度为0.50～500mg/L具有良好的线性关系(R2=0.9999),能对浒苔提取液中的叶绿素a、b进行准确定量。
A convenient and user-friendly reverse phase-high performance liquid chromatography （RP-HPLC） analysis method was established for the determination of chlorophyll a and chlorophyll b in Enteromorpha prolifera The following HPLC conditions are follows： Diamonsil-C18 （4.6 mm×150 mm） column is used; MeOH-0.2% acetic acid and acetone as the mobile phase with linear gradient elution are used; flow-rate is 0.8 mL/min, detection length of Vis is 430 nm; injection volume is 10μL. The results indicated that the developed method can be used for the determination of chlorophyll a and b in E. prolifera with a good resolution and linearity between 0.50 - 500 mg/L （R^2=0.999 9）.The method is specific, simple, fast, sensitive and feasible for the determination of chlorophyll a and chlorophyll b, which can eliminate the interference of chlorophyll derivatives in E. prolifera.
出处
《海洋科学》
CAS
CSCD
北大核心
2010年第2期23-27,共5页
Marine Sciences
基金
国家自然科学基金项目(20905017
20602009)
海洋公益性行业科研专项项目(200705011
200805039)
国家海洋局第一海洋研究所基本科研业务专项项目(GY-022008T32
GY-022008G47)
