摘要
采用高碘酸钠法,以BSA,OVA为载体蛋白合成斯皮诺素的免疫抗原和包被抗原,通过紫外扫描法鉴定,偶联率分别为17,13.7。采用间接ELISA和间接竞争ELISA测定小鼠血清,其最高效价达1∶32 000,灵敏度为211.6μg·L-1且交叉反应率低,表明小鼠经斯皮诺素-BSA免疫后可产生高效价、特异性好的抗斯皮诺素抗体。该实验首次成功制备了具有免疫原性的斯皮诺素人工抗原,为进一步制备斯皮诺素的单克隆抗体及建立斯皮诺素的免疫分析方法提供参考。
Immunogenic antigen（spinosin-BSA） and coating antigen（spinosin-OVA） of spinosin were synthesized by sodium periodate oxidation method. UV scanning analysis method showed that these two spinosins were successfully conjugated with carrier protein and the coupling ratio was 17 and 13. 7,respectively. Meanwhile,when immunized by spinosin-BSA,the mice can produce anti-spinosin antibodies with the high titer（1∶ 32 000）,specificity（IC50211. 6 μg·L-1） and low cross-reaction rate measured by ELISA tests.The artificial antigen of spinosin was successfully synthesized,which can be applied for preparation of monoclonal antibodies and establishment of appropriate immune method.
作者
章飚
李彦川
冯红
许宏亮
张兰兰
李萍
ZHANG Biao;LI Yan-chuan;FENG Hong;XU Hong-liang;ZHANG Lan-lan;LI Ping(State Key Laboratory of Natural Medicines,China Pharmaceutical University,Nanjing 210009,China;Digital Materia Medica Traditional Chinese Medicine Testing Co.,Ltd.,Baoding 071200,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2018年第13期2747-2750,共4页
China Journal of Chinese Materia Medica
基金
天津市科技计划项目(17YFCZZC00350)
关键词
斯皮诺素
高碘酸钠法
人工抗原
免疫分析
抗体
spinosin
sodium periodate oxidation method
artificial antigen
immunoassay method
antibody
