摘要
为研发具有自主知识产权的锡林郭勒盟牛源性检测试剂盒。以8种动物的线粒体全基因组序列为基础,通过比对分析筛选出牛特异的引物和探针组合进行荧光定量聚合酶链式反应(polymerase chain reaction,PCR)。以所设计的引物和探针为基础的牛源性成分检测具有高度的特异性(仅对牛源性样品有典型扩增曲线,对其他动物来源的样品无扩增曲线)。检测灵敏度试验表明,利用此引物和探针的检测方法具有高度的灵敏度,可以检测到1 pg级的模板DNA。牛肉加工制品中牛源性定量检测试验表明,所研发的引物和探针具有较好的定量检测能力。以上研究结果表明此引物和探针组合适用于牛源性成分的定性和定量检测。
To establish a detection kit of bovine origin which Xilin Gol League Independent Intellectual Property,the primers and probes were designed on the whole mitochondrial genome sequences from 8 animals.The result showed high specitivity,which no specific amplification for other kinds of animals except for bovine.The objective of this study was to design the primer and probe with intellectual property which can be used to detect bovine-derived materials by means of Taq Man PCR. The results inferred that the technique based on the primers and probe was specific for species identification and quantification,and was able to detect 1 pg template.This biotechnological method based on Taq Man PCR was appropriate to identify bovine-derived materials in farm products and foods.
作者
郭梁
徐伟良
钱俊平
郭元晟
海小
李春冬
雅梅
GUO Liang1,2,3, XU Wei-liang1,2, QIAN Jun-ping1,2,3, GUO Yuan-sheng1,2,3, HAI Xiao1,2,3, LI Chun-dong1,2, YA Mei1,2,3(1. Xilingol Vocational College, Xilinhot 026000, Inner Mongolia, China ; 2. Xilin Gol Institute of Bioengineering, Xilinhot 026000, Inner Mongolia, China; 3.Xilin Gol Food Testing and Risk Assessment Center, Xilinhot 026000, Inner Mongolia, Chin)
出处
《食品研究与开发》
CAS
北大核心
2018年第16期153-157,共5页
Food Research and Development
基金
内蒙古自治区高等学校科学研究项目(NJZY18369)
锡林郭勒生物工程研究院项目(201601
201602)
