摘要
实时荧光定量PCR技术作为一种DNA定量检测的工具,可应用于肉制品种类鉴别和定量分析中。相比于传统感官和理化的鉴别方法,该技术具有灵敏性高、特异性强、操作简便、省时省力等特点。使用Taq Man荧光探针和荧光染料可以直接测定PCR循环后产物的总量,确定扩增DNA片段的种类及数量,从而确定肉品种类及添加量。因此,可以将该技术应用到肉制品成分及安全的检测中。本文介绍了实时荧光定量PCR技术的基本原理,主要综述了该技术在肉品种类鉴定方面的应用,简述其在肉制品细菌污染检测的应用,并对该技术在肉类研究中的应用进行展望。
Quantitative real-time PCR as a technology for quantitative measurement of DNA has been widely used in the identification and quantitative analysis the species of meat products. Compared with the traditional or physical and chemical identification,quantitative real-time PCR has the advantages of high sensitivity and specificity, simple operation and time saving.The amount of products of the PCR cycle and types of amplified DNA fragments can be determined by the use of TaqMan probe and fluorescence dyes, then types and addition of meat is determined.Therefore, the technology can be applied to the detection of adulteration of meat and meat products safety.The principle of quantitative real-time PCR was introduced briefly, and application of quantitative real-time PCR was mainly reviewed in species identification and bacterial contamination of meat and meat products.Finally,the application of this technology in meat progress was prospected.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第17期324-328,334,共6页
Science and Technology of Food Industry
基金
国家自然科学基金(31471599)
国家重点研发计划项目(2016YFD0401504)
