摘要
【目的】为了探索食用级牛皮脱毛方法.【方法】采用碱-酶联合方法,以碱液浓度、碱发时间、酶解时间、酶解温度、酶液pH、酶添加量为研究因素,在单因素基础上,试验应用Plackett-Burman设计、响应面设计针对牛皮、牛蹄脱毛进行工艺参数优化,并建立二次回归方程.【结果】最佳脱毛工艺参数为:碱液浓度为6%,碱发时间为23h,碱性蛋白酶添加量为0.3%,酶解温度为53℃,酶液pH为10.2,酶解时间为3h.【结论】最佳脱毛参数条件下的牛蹄脱毛率为92.51%,牛皮脱毛率为94.01%,碱-酶联合脱毛的方法对于食用级脱毛具有一定应用价值.
【Objective】To explore the hair removal method of cattle leather and hoof for edible utilization.【Method】The alkali-enzyme combined procedure was designed with Plackett-Burman and response surface method.The factors affecting processing efficiency,including alkali concentration,alkali time,enzymolysis time,enzymolysis temperature,enzyme pH and enzyme amount,were selected and optimized and two relative regression equations were established.【Result】The optimal process parameters were as follows：6% of alkali solution concentration,23 hours of alkali treatment,0.3% of alkaline protease amount,53 ℃ of enzymolysis temperature,the pH 10.2 of enzyme solution,3 hours of enzymolysis time.【Conclusion】The hair removal rates of hoof and leather were 92.51% and 94.01 under optimized conditions respectively.The alkali and enzyme combination method for edible utilization of cattle leather and hoof was promising.
作者
王艺伦
张玉斌
余群力
刘金鑫
韩广星
吴磊
张文华
WANG Yi-lun;ZHANG Yu-bin;YU Qun-li;LIU Jin-xin;HAN Guang-xing;WU LEI;ZHANG Wen-hua(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China;Shandong Green Food Co.Ltd.,Linyi 276600,China;Hebei Fuchengwufeng Food Co.Ltd.,Sanhe 065201,China;Ningxia Xiahua Meat Food Co.Ltd.,Zhongwei 755000,China)
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2018年第3期150-157,共8页
Journal of Gansu Agricultural University
基金
国家现代农业产业技术体系项目(CARS-38)
甘肃省农业生物技术研究与应用开发项目(GNSW-2015-16)
关键词
牛皮
牛蹄
食用碱
碱性蛋白酶
脱毛率
cattle leather
cattle hoof
dietary alkali
alkaline protease enzyme
hair removal rate
