摘要
目的探究卵巢高级别浆液性癌组织中多发性骨髓瘤SET结构域蛋白(MMSET)表达及其对卵巢癌细胞侵袭迁移能力的影响。方法收集卵巢高级别浆液性腺癌组织45例份、正常卵巢组织27例份,分别应用RTPCR法、Western blotting法检测组织中MMSET表达。取人卵巢癌细胞系CAOV3及OVCAR3,分为si-NC组及siMMSET组,si-NC组转染空白质粒,si-MMSET组转染靶基因质粒。应用划痕实验、Transwell小室实验测定卵巢癌细胞迁移、侵袭水平,Western blotting法检测P-AKT、AKT蛋白表达。结果卵巢高级别浆液性癌组织中MMSET表达高于正常卵巢组织(P<0.05);MMSET表达与患者淋巴结转移、腹水量及CA125水平有关(P均<0.05);si-NC组及si-MMSET组CAOV3卵巢癌细胞MMSET相对表达量分别为0.9586±0.1472,0.4454±0.0516,OVCAR3卵巢癌细胞MMSET相对表达量分别为0.8307±0.0796,0.5304±0.0670,两组比较,差异有统计学意义(P均<0.05)。划痕实验结果显示,48 h后卵巢癌细胞CAOV3si-NC组及si-MMSET组的划痕面积变化率分别为47.21%±5.38%、22.22%±6.20%;卵巢癌细胞OVCAR3si-NC组及si-MMSET组的划痕面积变化率分别为57.52%±2.76%、28.03%±2.48%,两组比较,差异有统计学意义(P均<0.05)。Transwell小室实验结果显示,卵巢癌细胞CAOV3 si-NC组及si-MMSET组的穿膜细胞数分别为(27±4)、(10±2)个,卵巢癌细胞OVCAR3 si-NC组及siMMSET组的穿膜细胞数分别为(70±5)、(33±3)个,两组比较,P均<0.05。si-NC组及si-MMSET组卵巢癌细胞CAOV3中P-AKT蛋白相对表达量分别为0.457 1±0.152 8、0.223 9±0.121 0,卵巢癌细胞OVCAR3中P-AKT蛋白相对表达量分别为0.657 0±0.106 8、0.439 9±0.100 2,两组比较,差异有统计学意义(P均<0.05)。结论卵巢癌高级别浆液性癌组织中MMSET呈过表达,过表达的MMSET能促进卵巢癌细胞侵袭迁移。
Objective To observe the expression of multiple myeloma SET domain（ MMSET） protein in the highgrade serous ovarian cancer and its effect on the invasion and metastasis of ovarian cancer cells. Methods RT-PCR and Western blotting was performed to determine the expression of MMSET in 45 cases of high-grade serous ovarian cancer tissues and 27 cases of normal ovarian tissues. The human ovarian cancer cell lines CAOV3 and OVCAR3 were divided into the si-NC group and si-MMSET group. The cells in the si-NC group were transfected with blank plasmid,and the siMMSET group with target gene plasmid. The migration and invasion abilities of ovarian cancer cells were detected by Scratch test and Transwell chamber experiment. Western blotting was used to detect the protein expression of P-AKT and AKT. Results The expression level of MMSET in the high-grade serous ovarian cancer tissues was significantly higher than that in the normal ovarian tissues and was related to the lymph node metastasis,abdominal water volume,and CA125 level（ all P〈0. 05）. The relative expression levels of MMSET of CAOV3 cells in the si-NC group and si-MMSET group were 0. 958 6 ± 0. 147 2 and 0. 445 4 ± 0. 051 6,respectively,with significant difference（ both P〈0. 05）; the relative expression levels of MMSET of OVCAR3 ells in the si-NC group and si-MMSET group were 0. 830 7 ± 0. 079 6 and 0. 530 4± 0. 067 0,respectively,with significant difference（ both P〈0. 05）. The Scratch test results showed that the rates of change in the area of scratches of ovarian cancer cells CAOV3 at 48 h in the si-NC group and si-MMSET group were47. 21% ± 5. 38% and 22. 22% ± 6. 20%,respectively; the rates of change in the area of scratches of ovarian cancer cells OVCAR in the si-NC group and si-MMSET group were 57. 52% ± 2. 76% and 28. 03% ± 2. 48%,respectively,with significant difference（ both P〈0. 05）. Transwell test showed that the transmembrane cells of ovarian cancer cells CAOV3 in the si-NC group and si-MMSET group were 27 ± 4 and 10 �
作者
陈颖
刘鑫慧
毕芳芳
杨清
CHEN Ying;LIU Xinhui;BI Fangfang;YANG Qing(Shengjing Hospital of China Medical University,Shenyang 110004,China)
出处
《山东医药》
CAS
2018年第25期23-26,共4页
Shandong Medical Journal
基金
辽宁省卵巢癌恶性肿瘤病例信息平台的建立及诊治技术规范化推广项目(LNCCC-A01-2015)
中国医科大学2017年度学科提升计划项目(2017CXTD05)
沈阳市科技计划项目(17-230-9-10)
盛京自由研究者计划项目(201704)
