摘要
目的探讨Yes相关蛋白、p63和Bcl-2蛋白在弥漫大B细胞淋巴瘤(DLBCL)组织中表达的意义及相互关系。方法应用免疫组化GTVision二步法检测45例DLBCL和25例反应性淋巴组织增生(RHL)中Yes相关蛋白、p63和Bcl-2蛋白的表达。结果 Yes相关蛋白在DLBCL中的表达阳性率为60.0%,在RHL中的表达阳性率为100%,差异具有统计学意义(P<0.05)。在DLBCL中,p63、Bcl-2蛋白的表达阳性率分别为44.4%和82.2%;在RHL中,p63的表达阳性率为0,而Bcl-2蛋白阳性细胞主要分布于套区和副皮质区,滤泡生发中心为阴性,均为正常表达模式,未见异常表达,p63、Bcl-2蛋白在DLBCL和RHL两组间表达差异具有统计学意义(P<0.05)。在DLBCL中,Yes相关蛋白、p63和Bcl-2蛋白表达之间两两比较均无相关性(P>0.05),且与年龄、性别、发病部位和免疫分型等临床病理特征无关(P>0.05)。结论 Yes相关蛋白、p63和Bcl-2蛋白与DLBCL的发生发展有关,其中Yes相关蛋白可能扮演抑癌基因的角色。
Objective To investigate the expression of Yes-associated protein,p63 and Bcl-2 in diffuse large B cell lymphoma. Methods Immunohistochemistry was used to detect the expression of Yes-associated protein,p63 and Bcl-2 in 45 cases of diffuse large B cell lymphoma（ DLBCL） and 25 cases of reactive hyperplasia of lymph node（ RHL）. Results The positive expression of Yes-associated protein in DLBCL and RHL was 60. 0%and 100. 0%,respectively. The difference between the two groups was statistically significant（ P〈0. 05）. The positive expression of p63 and Bcl-2 in DLBCL were 44. 4% and 82. 2%,respectively. In RHL,p63 was not expressed,and Bcl-2 was not abnormally expressed（ only positive for mantle and parafollicular while negative for germinal center）. The expression of p63 and Bcl-2 showed significant difference between the two groups（ P〈0. 05）. The expression of Yes-associated protein,p63 and Bcl-2 in DLBCL were neither correlated with each other（ P〈0. 05）,nor with clinico-pathological features（ P〈0. 05）. Conclusion Yes-related proteins,p63 and Bcl-2 proteins are associated with the development of DLBCL,and Yes-related proteins may play a role as the tumor suppressor gene.
作者
沈艳
何新明
林喜娜
陈贵东
杨通
古婉仪
顾霞
Shen Yan 1,He Xinming 2,Lin Xina 2,Chen Guidong 1,Yang Tong 1,Gu Wanyi 1,Gu Xia 2(1.Department of Pathology,The Second Affiliated Hospital of Gugangzhou Medical University,Guangzhou Guangdong 511447,China; 2.Department of Pathology,The First Affiliated Hospital of Gugangzhou Medical University,Guangzhou Guangdong 510120,China)
出处
《遵义医学院学报》
2018年第3期314-319,共6页
Journal of Zunyi Medical University
基金
广州市卫生局医药卫生科技项目(NO:20161A010100)
