摘要
目的:研究脑出血大鼠模型中血脑屏障通透性变化与细胞凋亡、炎症及应激反应激活的相关性。方法:选择SD大鼠作为实验动物,并分为脑出血组和对照组,造模24h后按照甲酰胺浸泡法测定血肿周围脑组织中血脑屏障通透性参数的水平以及细胞凋亡分子、炎症反应分子、氧化应激分子的含量。结果:脑出血组大鼠血肿周围脑组织中血脑屏障通透性参数EB水平显著高于对照组,DKK1、PAR-1、Bax、Cyt-C、NF-κB、ICAM1、VCAM1、TNF-α、MMP9、NOX4、MDA的含量高于对照组,β-catenin、Bcl-2、Mn-SOD、GSH、T-AOC的含量低于对照组;Pearson相关性分析显示:脑出血组大鼠血肿周围脑组织的EB水平与DKK1、PAR-1、Bax、Cyt-C、NF-κB、ICAM1、VCAM1、TNF-α、MMP9、NOX4、MDA的含量呈正相关,与β-catenin、Bcl-2、Mn-SOD、GSH、T-AOC的含量呈负相关。结论:脑出血大鼠模型中血脑屏障通透性的增加与细胞凋亡、炎症及应激反应的过度激活有关。
Objective：To study the correlation of the changes of blood brain barrier permeability with the activation of apoptosis,inflammatory response and stress response in rats with cerebral hemorrhage.Methods：SD rats were chosen as experimental animals and divided into the cerebral hemorrhage group and the control group.24 hours after model making,formamide soaking method was referred to determine the levels of blood brain barrier permeability parameters as well as the contents of apoptosis molecules,inflammatory response molecules and oxidative stress molecules in brain tissues around the hematoma.Results：Blood brain barrier permeability parameter EB level in brain tissues around hematoma of cerebral hemorrhage group was significantly higher than that of control group（P〈0.05）,DKK1,PAR-1,Bax,Cyt-C,NF-κB,ICAM1,VCAM1,TNF-α,MMP9,NOX4 and MDA contents were higher than those of control group,andβ-catenin,Bcl-2,Mn-SOD,GSH and T-AOC contents were lower than those of control group（P〈0.05）;Pearson correlation analysis showed that EB level in brain tissues around hematoma of cerebral hemorrhage group was positively correlated with DKK1,PAR-1,Bax,Cyt-C,NF-κB,ICAM1,VCAM1,TNF-α,MMP9,NOX4 and MDA contents,and negatively correlated withβ-catenin,Bcl-2,Mn-SOD,GSH and T-AOC contents.Conclusion：The increase of blood brain barrier permeability in rats with cerebral hemorrhage is related to the excessive activation of apoptosis,inflammatory response and stress response.
作者
周喜燕
于淼
崔换新
ZHOU Xi-yan;YU Miao;CUI Huan-xin(The Affiliated Qingdao Hiser Hospital of Qingdao University,Qingdao 266000,China)
出处
《海南医学院学报》
CAS
2018年第14期1299-1302,共4页
Journal of Hainan Medical University
基金
山东省中医药科技发展计划项目(2015-356)~~
关键词
脑出血
血脑屏障
细胞凋亡
炎症反应
应激反应
Cerebral hemorrhage
Blood brain barrier
Apoptosis
Inflammatory response
Stress response
