摘要
目的了解成都地区诺如病毒各基因型的流行情况,探索疾病暴发增长的原因,为疾病预防控制工作提供科学的依据。方法选取2014-2016年间,采用荧光PCR方法检测阳性的部分诺如病毒标本,对衣壳蛋白VP1基因的ORF2区进行测序,构建进化树并进行同源性分析。结果 89份标本均为诺如病毒GII群,包括GII.1、GII.2、GII.3、GII.6、GII.13和GII.17 6种基因型。其中2014-2015年以GII.17型为主,从2016年起,GII.2型逐渐取代GII.17型成为了主要流行株。结论成都地区诺如病毒流行株的基因型变化显著,导致了疾病的暴发增长,增大了疾病防控的难度。需要持续开展诺如病毒的分子流行病学研究,以掌握病毒变异情况,预测疾病可能的流行趋势,提高疾病防控的预警能力。
Objective To understand the prevalence of norovirus genotypes in Chengdu,explore the causes of outbreaks and provide a scientific basis for prevention and control of the disease. Methods The study was conducted on part samples confirmed to be norovirus positive by fluorescent PCR during 2014 to 2016; the ORF2 region of VP1 was sequenced,and an evolution tree was constructed,followed by homology analysis.Results Norovius in all of the 89 samples analyzed was identified as GII genogroup,covering six genotypes including GII. 1,GII. 2,GII. 3,GII. 6,GII. 13 and GII. 17. The GII. 17 was the main genotype from 2014 to2015. Since 2016,GII. 2 has replaced GII. 17 as the dominant genotype. Conclusion Norovirus genotypes in Chengdu have varied considerably,increasing the incidence rate and adding to the difficulty in prevention and control of the disease. It is necessary to continue the molecular epidemiological studies on norovirus to understand the variation of norovirus genotypes,predict epidemic trends and improve the early warning ability.
作者
程悦
孟建彤
张晓春
黄韦唯
陈振华
李广垣
黄薇
陈恒
CHENG Yue;MENG Jiantong;ZHANG Xiaochun;HUANG Weiwei;CHEN Zhenhua;LI Guangyuan;HUANG Wei;CHEN Heng(Chengdu Center for Disease Control and Prevention,Chengdu 610041,Sichuan Province,China;Meishan Center for Disease Control and Prevention,Chengdu 620000,Sichuan Province,China.)
出处
《预防医学情报杂志》
CAS
2018年第10期1294-1297,共4页
Journal of Preventive Medicine Information
关键词
诺如病毒
基因型
进化分析
norovirus
genotype
evolution analysis
