摘要
目的了解2016—2017年盐城市手足口病肠道病毒谱分布特征,并对鉴定为CVA10型肠道病毒VP1基因进行分子进化特征分析。方法疑似手足口病咽、肛拭子标本,采用实时荧光RT-PCR方法检测肠道病毒核酸,以RT-PCR法扩增CVA10型VP1基因全长编码区并进行序列分析,采用相应的生物信息软件进行核苷酸及氨基酸序列比对及基因种系进化特征分析。结果 2016—2017年盐城市手足口病原主要以其他肠道病毒为主,其他肠道病毒的流行共有10种基因型,其中CVA6、CVA10型为优势流行株,占其他肠道病毒阳性的77.03%、13.51%。19株CVA10VP1区核苷酸、氨基酸同源性分别为92.5%~100.0%、97.3%~100.0%。2年间,毒株组内核苷酸同源性分别为96.4%~100.0%、92.5%~100.0%;氨基酸同源性分别为98.0%~100.0%、97.7%~100.0%。遗传进化树显示:盐城市CVA10代表株属于C3、C4基因亚群的毒株,盐城代表株364/JSYC/2017与2014年云南代表株(LC013415)聚集成簇单独构成C3基因亚群分支,其余18株盐城代表株属于C4基因亚群分支,为主流优势流行株。结论 2016—2017年盐城市手足病病原由多种基因型的肠道病毒共同流行,CVA10型为其他肠道病毒的优势流行株。开展CVA10型肠道病毒分子流行病学研究,了解毒株进化变异趋势,对防控手足口病具有重要意义。
Objective To study the pathogenic spectrum of the enteroviruses for hand,foot and mouth disease（HFMD）in Yancheng city from 2016 to 2017;to analyze the genetic evolution characteristics of the VP1 gene of coxsackievirus A 10（CVA10）.Methods Specimens of pharyngeal and anal swabs from suspected HFMD were collected.Real time RT-PCR was used to detect nucleic acid of enteroviruses;RT-PCR was used to amplify VP1 coding region followed by sequencing analysis.The nucleotide and protein sequences were subjected to alignment and phylogenetic analysis by bioinformatics software.Results The dominant prevalent strains of the enterovirus were other enteroviruses in Yancheng city from 2016 to 2017,categorized to 10 genotypes.Among which,CVA6 and CVA10 were dominant epidemic genotypes,accounted for 77.03%、13.51% of other enteroviruses,respectively.The nucleotide and amino acid homology of 19 CVA10 VP1 regions were 92.5%-100.0%and 97.3%-100.0%,respectively.In the past 2 years,the homology of the nucleotide in same genetic group was 96.4%-100.0% and 92.5%-100.0%,respectively;the amino acid homology was 98.0%-100.0% and 97.7%-100.0%,respectively.Phylogenetic analysis showed that the representative strains of CVA10 belonged to C3,C4 evolutionary sub-branches.The Yancheng representative strain 364/JSYC/2017 clustered with the Yunnan representative strain（LC013415）to C3 evolutionary sub-branch.The other 18 strains belonged to C4 evolutionary sub-branch,which was the dominant sub-branch.Conclusion The spectrum of pathogens causing HFMD epidemics in Yancheng city from 2016 to 2017 had a variety of genotypes and CVA10 was one of the dominant epidemic genotype among other enteroviruses.It was important to study the molecular epidemiology of genotype CVA10 in order to prevent and control the HFMD epedemics.
作者
朱根忠
陈国清
王瑶
李峰
徐士林
李长城
邵荣标
ZHU Gen-zhong;CHEN Guo-qing;WANG Yao;LI Feng;XU Shi-lin;LI Chang-cheng;SHAO Rong-biao(Jianhu County Center for Disease Control and Prevention,Jiangsu Yancheng 224000,Chin)
出处
《江苏预防医学》
CAS
2018年第4期371-373,381,共4页
Jiangsu Journal of Preventive Medicine
基金
盐城市医学科技发展计划项目(YK2015032)
关键词
手足口病
CVA10
VP1基因
序列分析
分子流行病学
Hand,foot and mouth disease （HFMD）
CVA10
the VP1 gene
Sequence analysis
Molecular epidemiology
