摘要
采用超声浸提法提取了虎杖多糖,选择料液比、提取温度和提取时间作为单因素实验,利用响应曲面法优化其提取工艺,并应用红外光谱检测多糖的理化特征。结果显示虎杖多糖提取的最优条件为:料液比1∶25(g/m L),提取温度81℃、提取时间101 min。在此条件下虎杖多糖提取率理论值为192.46 mg/g,实测值为194.88 mg/g。优化后的工艺是提取虎杖多糖简单高效的方法,有利于其在药品及保健食品中的广泛应用。
Polygonum cuspidatum polysaccharides were extracted by ultrasonic, solid - liquid ratio, extractiontemperature and extraction time were selected as a single factor, and response surface methodology was applied to optimizethe extraction conditions. The physicochemical characteristics of Polygonum cuspidatum polysaccharides were furtherstudied by FTIR. The results showed that optimum extraction conditions were as follows： the ratio of material to liquid was125 （g / mL）, the extraction temperature was 81 ℃ and the extraction time was 101 min. Under the above conditions,the prediction yield of polysaccharide yield was 192. 46 mg / g and verifying result was 194. 88 mg / g. The optimumextraction conditions were simple and efficient. It would be helpful for the application of Polygonum cuspidatum in foodand pharmaceutical industries.
作者
王佳
李进霞
刘斌焰
赵一锦
冯碧阳
WANG Jia, LI Jin-xia, LIU Bin-yan, ZHAO Yi-jin, FENG Bi-yang(Datong University, Shanxi Datong 037009, Chin)
出处
《广州化工》
CAS
2018年第15期96-99,共4页
GuangZhou Chemical Industry
基金
山西大同大学青年科研基金资助项目(No:2016Q7)
大同市应用基础研究计划项目(2018153)
关键词
虎杖多糖
响应曲面法
理化特征
Polygonum cuspidatum polysaccharides
response surface methodology
physicochemical characteristics
