摘要
目的研究依鲁替尼对急性T淋巴细胞白血病细胞CCRF-CEM细胞株增殖抑制的作用。方法使用不同浓度的依鲁替尼作用于CCRF-CEM(人急性T淋巴细胞白血病细胞)细胞株,分别作用24、48、72 h后,采用CCK8检测细胞抑制率,采用Annexin V-FITC/PI荧光双染色测定细胞的凋亡率,观察依鲁替尼对CCRF-CEM的凋亡影响。结果不同浓度依鲁替尼作用CCRF-CEM细胞后,细胞增殖抑制率明显升高(P<0.01)并且抑制率随药物浓度增高而增高。在同一浓度依鲁替尼作用细胞后,不同的作用时间,抑制率也有明显的变化(P<0.01),呈时间依懒性,但在低浓度下,72h细胞的增殖抑制率比48h低。依鲁替尼作用细胞后,细胞凋亡率明显升高(P<0.01),并且呈药物浓度依赖性。结论依鲁替尼可以诱导T淋巴细胞CCRF-CEM凋亡,并且依赖药物浓度和作用时间。
Objective To study the effect of ibrutinib on proliferation inhibition of CCRF-CEM cell lines in acute T lymphocytic leukemia cells.Methods Different concentrations of ibrutinib were used to act on CCRF-CEM（human acute T-lymphocytic leukemia） cell lines.After 24,48,and 72 h,CCK8 was used to determine the cell inhibition rate,respectively.The apoptosis rate of cells was measured by Annexin V-FITC/PI double staining and the effect of ibrutinib on the apoptosis of CCRF-CEM was observed.Results After CCRF-CEM cells were treated with different concentrations of ibrutinib,the inhibition rate of cell proliferation was significantly increased（P〈0.01）,and the inhibition rate increased with the increase of drug concentration.After the cells were treated with ibrutinib at the same concentration,the inhibition rate was also changed significantly（P〈0.01）.The inhibition time was time-dependent,but at low concentrations,the 72 h cell proliferation inhibition rate was lower than that of 48 h.After ibrutinib action on cells,the apoptosis rate was significantly increased（P〈0.01）,and was drug-dependent.Conclusion Ibrutinib can induce apoptosis of T lymphocytes CCRF-CEM,and depends on drug concentration and duration of action.
作者
黄小勇
史海燕
李宝莉
HUANG Xiao-yong,SHI Hai-yan,LI Bao-li(Medicine College of Yan＇an University, Yan＇an 716000, China)
出处
《延安大学学报（医学科学版）》
2018年第2期16-19,共4页
Journal of Yan'an University:Medical Science Edition
基金
延安大学2015年校级项目(No:YDK2015-54)
