摘要
采用高效液相色谱法测定保健食品红曲片中β-谷甾醇的含量。色谱条件为:C18柱色谱柱,4.6 mm×250 mm,5μm;流动相乙腈-异丙醇=70∶30(V/V);流速1.0 m L/min;检测波长210 nm。测定样品中β-谷甾醇含量的RSD为0.8%(n=6),回收率范围为98.4%~100.3%。该方法简便、快速、准确,适用于测定保健食品红曲片中β-谷甾醇的含量。
To determine the contents of β-sitosterol in monascus piece of health foods by HPLC.The chromatographic conditions included chromatographic column（C18 column,4.6 mm×250 mm,5 μm） with UV detection at 210 nm and the mobile phase consisted of acetonitrile-isopropanol=70∶30（V/V）with the flow rate at 1.0 m L/min.The RSD of β-sitosterol in monascus piece of health foods was 0.8%（n=6） and the recovery was 98.4%-100.3%.This method is simple,rapid and accurate,so it can be used for the determination of β-sitosterol content in monascus piece of health foods.
作者
魏嘉良
杨学灵
陈爱民
郑创政
蔡子洋
许青
WEI Jia-ling;YANG Xue-ling;CHEN Ai-min;ZHENG Chuang-zheng;CAI Zi-yang;XU Qing(Guangzhou Zhongke Testing Services Co.,Ltd.,Guangzhou 510650,China)
出处
《广州化学》
CAS
2018年第3期52-55,共4页
Guangzhou Chemistry
