摘要
目的:建立秋海棠属药材中芦丁的含量测定方法,为完善秋海棠属药材的质量标准提供理论依据。方法:采用HPLC,色谱柱Diamonsil(钻石)-C18(4.6 mm×250 mm,5μm);流动相甲醇-0.5%磷酸溶液梯度洗脱;柱温30℃;检测波长257nm。结果:秋海棠属药材因种类、产地及药用部位不同,其芦丁的含量存在差异,以贵州开阳南江峡谷产盾叶秋海棠地上部分中芦丁的含量为最高;不同部位中芦丁的含量分布为叶、茎>根茎。结论:方法简便易行、重复性好、结果准确,可作为秋海棠属药材中芦丁的含量测定方法。秋海棠药材入药建议以地上部分更为合理。
Objective：To consummate the quality standard and provide theory evidence of Begonia herbs, by constituting the method of assaying of rutin in Begonia herbs. Method： The content of rutin of Begonia L. herbs were determined by HPLC. Diamonsil Chromatographic column（Diamond）-C18 （4.6 mm × 250 mm,5 μm）. Mobile phase was methanol-0.5% phosphoric acid gradient elution. Column temperature was 30℃. The detection wavelength 257 nm. Result： The content of rutin was influenced by different species, different origins and different medicinal parts, and the rutin content of leaf, stem which was higher than root . Among them, The content of rutin was the highest than others, which of Begonia cavalerei in Kaiyang Nanjiang canyou of Guizhou. Conclusion： The method by constituting is simple and convenient, reproducible,accurate. It can conduct method of assaying of rutin in Begonia herbs. It is right to collect the leaf and stem part of the herbs which provide a reliable basis for conservation and reasonable development of Begonia herbs resources in Guizhou.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第1期40-42,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30660224)
