摘要
背景:研究发现接骨木根皮能通过促进成骨细胞的分裂增殖、分泌碱性磷酸酶加快骨基质钙化,促使新骨形成的同时也具有促进坏死骨的吸收作用。目的:观察新疆西伯利亚接骨木树皮2种提取物促进大鼠骨折愈合的影响。方法:将108只3月龄雄性SD大鼠随机分为假手术组,模型组,中华跌打丸组(剂量为0.54 g/kg,阳性对照),醇提物高、中、低剂量组(剂量分别为0.314,0.157,0.078 g/kg),水提物高、中、低剂量组(剂量分别为0.340,0.170,0.085 g/kg)。除假手术组外其他各组大鼠截断股骨干中段,制作股骨开放性骨折模型,假手术组仅暴露股骨干,不做截骨。造模后第1天开始灌胃不同药物到6周(1次/d)。第2,4,6周后观察西伯利亚接骨木2种提取物对血清相关生化指标、X射线片、生物力学及苏木精-伊红染色的影响。实验方案经新疆医科大学动物实验伦理委员会批准。结果与结论:①血清生化指标:各期醇提物高剂量组血钙低于假手术组、模型组,表明醇提物高剂量组成骨速度快,其次为醇提物中、低剂量组、水提物高剂量组;醇提物高剂量血磷高于假手术组、模型组、中华跌打丸组;各期醇提物高剂量组血碱性磷酸酶显著升高于其他组,表明醇提物高剂量组成骨细胞活动旺盛,其次为水提物高剂量组;②X射线片:大鼠骨折区的愈合醇提物高剂量组显著,中华跌打丸组、水提物高、中剂量组,醇提物中剂量组良好,醇提物低剂量组、水提物低剂量组欠佳;③三点弯曲力学试验:2种提取物各组6周大鼠股骨最大载荷、最大挠度值均高于模型组,醇提物高剂量组明显升高,其次为水提物高、中剂量组,醇提物中剂量组;④苏木精-伊红染色:随时间的延长骨折区逐渐形成纤维性骨痂,骨髓腔沟通,醇提物高剂量组、水提物高剂量组骨性愈合好于其他组;⑤结果表明,2种提取物具有很好的促进骨折愈合的作用,�
BACKGROUND:Studies have found that Sambucus sibirica Nakai can promote osteoblast division,proliferation,and secretion of alkaline phosphatase to accelerate bone matrix calcification,promote new bone formation,and promote the absorption of necrotic bone.OBJECTIVE:To observe the effects of two extracts of Xinjiang Sambucus sibirica Nakai bark in promoting fracture healing in rats.METHODS:A total of 108 male Sprague-Dawley rats,3 months old,were randomly divided into sham-operated group,model group,Zhong Hua Die Da Wan group(0.54 g/kg,negative control group),high-,middle-and low-dose ethanol extract groups(0.314,0.157,0.078 g/kg),high-,middle,and low-dose water extract groups(0.340,0.170,0.085 g/kg).Except for the sham-operated group,the middle segment of the femoral shaft was cut off to make a model of open femoral fracture.In the sham-operated group,only the femoral shaft was exposed and no osteotomy was performed.Intragastric administration began at 1 day after modeling and continued for 6 weeks(1 time/day).At 2,4,6 weeks after modeling,the effects of two extracts of Sambucus sibirica Nakai bark on serum-related biochemical indicators,X-ray imaging,biomechanical indicators and hematoxylin-eosin staining were observed.RESULTS AND CONCLUSION:(1)Serum biochemical indicators:The serum calcium level of the high-dose ethanol extract group in each phase was lower than that of the sham-operated group and the model group,indicating that a faster osteogenesis in the high-dose ethanol extract group,followed by middle-dose ethanol extract group,low-dose ethanol extract group,and high-dose water extract group.The serum phosphorus level of ethanol extract groups was higher than that of sham-operated group,model group,and Zhong Hua Die Da Wan group;the serum alkaline phosphatase level of high-dose ethanol extract group at each stage was significantly higher than the other groups,indicating that a stronger osteoblast activity in the high-dose ethanol extract group,followed by the high-dose water extract group.(2)X-ray film:Frac
作者
吾兰·赛塔合买提
艾力江·阿斯拉
努尔哈那提·沙依兰别克
努尔曼·衣西坚
阿斯哈提·努尔买买提
方锐
Wulan·Saitahemaiti;Ailijiang·Asila;Nuerhanati·Shayilanbieke;Nuerman·Yixijian;Asihati·Nuermaimaiti;Fang Rui(College of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830011,Xinjiang Uygur Autonomous Region,China;Department of Joint Surgery,Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830011,Xinjiang Uygur Autonomous Region,China;Department of Orthopedics,First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,Xinjiang Uygur Autonomous Region,China;Fourth Department of Orthopedics,Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830011,Xinjiang Uygur Autonomous Region,China)
出处
《中国组织工程研究》
CAS
北大核心
2020年第20期3122-3129,共8页
Chinese Journal of Tissue Engineering Research
基金
新疆医科大学研究生创新创业启动基金项目(CXCY2018034)，项目负责人：吾兰·赛塔合买提~~
关键词
西伯利亚接骨木
醇提法
水提法
骨折愈合
Sambucus sibirica Nakai
ethanol extraction
water extraction
fracture healing
