摘要
目的:建立同时测定金蝉止痒胶囊中盐酸小檗碱、黄芩苷和蛇床子素含量的方法。方法:采用高效液相色谱法。色谱柱为Hypersil BDS C18,流动相为甲醇-乙腈-0.1%磷酸溶液-三乙胺(50∶30∶19∶1,V/V/V/V),流速为1.0 mL/min,检测波长为265 nm(盐酸小檗碱)、280 nm(黄芩苷)、322 nm(蛇床子素),柱温为30℃,进样量为10μL。结果:盐酸小檗碱、黄芩苷和蛇床子素检测进样量线性范围分别为80~800 ng(r=0.999 8)、60~600 ng(r=0.999 9)、60~600 ng(r=0.999 6);定量限分别为80、60、60 ng,检测限分别为24、20、20 ng;中间精密度、稳定性、重复性试验的RSD均小于2.0%;加样回收率分别为97.4%~98.3%(RSD=0.33%,n=6)、98.4%~99.6%(RSD=0.42%,n=6)、96.9%~99.0%(RSD=0.92%,n=6);耐用性试验的RSD均小于1.2%。结论:该方法操作简便、准确,精密度、稳定性、重复性、耐用性好,可用于金蝉止痒胶囊中盐酸小檗碱、黄芩苷和蛇床子素含量的同时测定。
OBJECTIVE：To establish the method for simultaneous determination of the content of berberine hydrochloride,baicalin and osthole in Jinchan zhiyang capsules. METHODS：HPLC method was adopted. The determination was performed in Hypersil BDS C18 column with mobile phase consisted of methanol-acetonitrile-0.1% phosphoric acid-three triethylamine（50∶30∶19∶1,V/V/V/V）at the flow rate of 1.0 mL/min. The detection wavelengths were set at 265 nm（berberine hydrochloride）,280 nm（baicalin）and 322 nm（osthole）. The column temperature was set at 30 ℃,and sample size was 10 μL. RESULTS：The linear range of berberine hydrochloride,baicalin and osthole were 80-800 ng（r=0.999 8）,60-600 ng（r=0.999 9）,60-600 ng（r=0.999 6）,respectively. RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The limits of quantitation were 80,60,60 ng,respectively,and the limits of detection were 24,20,20 ng,respectively. The recovery rates were 97.4%-98.3%（RSD=0.33%,n=6）,98.4%-99.6%（RSD=0.42%,n=6）and 96.9%-99.0%（RSD=0.92%,n=6）,respectively. RSDs of durability tests were all lower than 1.2%. CONCLUSIONS：The method is simple,accurate,precise,stable,reproducible and durable. It can be used for simultaneous determination of berberine hydrochloride,baicalin and osthole in Jinchan zhiyang capsules.
作者
黄传俊
梅勇
杨莉
罗磊
曾博程
龙涛
袁开超
谯志文
陈小雪
HUANG Chuanjun1,2,MEI Yong3,YANG Li3,LUO Lei3,ZENG Bocheng3,LONG Tao3,YUAN Kaichao3,QIAOZhiwen3,CHEN Xiaoxue3(1. Dept. of Pharmaceutical Affair, Hubei Provincial Hospital of TCM,Wuhan430061,China;2. Hubei Institute of TCM,Wuhan 430074,China;3. Chongqing CISCO PharmaceuticalResearch Institute Co.,Ltd.,Chongqing 401520,Chin)
出处
《中国药房》
CAS
北大核心
2018年第12期1621-1624,共4页
China Pharmacy
基金
重庆市青年科技人才培养计划项目(No.cstc2014kjrcqnrc10005)
